Spatially resolved shear distribution in microfluidic chip for studying force transduction mechanisms in cells

被引：36
|
作者：
Wang, Jianbin [2 ]
Heo, Jinseok [1 ,2 ]
Hua, Susan Z. [1 ,2 ]
机构：
[1] SUNY Buffalo, Dept Physiol & Biophys, Buffalo, NY 14260 USA
[2] SUNY Buffalo, Dept Mech & Aerosp Engn, Buffalo, NY 14260 USA
来源：
LAB ON A CHIP | 2010年 / 10卷 / 02期
基金：
美国国家科学基金会;
关键词：
EPITHELIAL-CELLS; COLLECTING DUCT; SUPPORT-SYSTEM; PRIMARY CILIUM; FLOW; CULTURE; POLYCYSTIN-2; ADHESION; STRESS;
D O I：
10.1039/b914874d
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluid shear stress has profound effects on cell physiology. Here we present a versatile microfluidic method capable of generating variable magnitudes, gradients, and different modes of shear flow, to study sensory and force transduction mechanisms in cells. The chip allows cell culture under spatially resolved shear flow conditions as well as study of cell response to shear flow in real-time. Using this chip, we studied the effects of chronic shear stress on cellular functions of Madin-Darby Canine Kidney (MDCK), renal epithelial cells. We show that shear stress causes reorganization of actin cytoskeleton, which suppresses flow-induced Ca2+ response.
引用
收藏
页码：235 / 239
页数：5
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