Characterization and mechanism of Mn(II)-based mixotrophic denitrifying bacterium (Cupriavidus sp. HY129) in remediation of nitrate (NO3--N) and manganese (Mn(II)) contaminated groundwater

被引：76
作者：
Bai, Yihan [1 ]
Su, Junfeng [1 ,2 ]
Wen, Qiong [1 ]
Huang, Tinglin [1 ,2 ]
Chang, Qiao [1 ]
Ali, Amjad [1 ]
机构：
[1] Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Peoples R China
[2] Xian Univ Architecture & Technol, Shaanxi Key Lab Environm Engn, Xian 710055, Peoples R China
来源：
JOURNAL OF HAZARDOUS MATERIALS | 2021年 / 408卷
基金：
中国国家自然科学基金;
关键词：
Cupriavidus sp. HY129; Denitrification; Extracellular polymeric substance; Mn(II)-oxidation; Mn(III)-intermediates; Multicopper oxidase; EXTRACELLULAR POLYMERIC SUBSTANCES; MICROBIAL COMMUNITY STRUCTURE; AUTOTROPHIC DENITRIFICATION; HEAVY-METAL; START-UP; REMOVAL; WASTE; DEGRADATION; IRON; EPS;
D O I：
10.1016/j.jhazmat.2020.124414
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The co-contamination of groundwater with nitrate (NO3--N) and manganese (Mn(II)) is a global issue that needs to be efficiently remediated. In this research, a novel denitrifying and manganese-oxidizing strain HY129 was isolated from the sediments sample of a drinking water and identified as Cupriavidus sp. HY129. The remediation ability of strain HY129 regarding the nitrate and Mn(II) pollution were investigated. The removal efficiency of nitrate and Mn(II) were 99.81% (0.229 mgL(-1) h(-1)) and 87.24% (0.233 mgL(-1) h(-1)) in bacterial culture after 72 h, respectively. Moreover, the addition of Mn(II) significantly enhanced the denitrification process, while excessive concentration of Mn(II) caused more NO2--N accumulation. The impacts of adsorption and oxidation activity on Mn(II) removal were investigated. Protein in extracellular polymeric substance (EPS) which produced in the Mn-oxidizing process was speculated to be the main cause of extracellular adsorption of Mn(II). Characterization of biogenic manganese oxides (BMO) confirmed the formation of high-valent manganese and the trapping experiment with sodium pyrophosphate (NaPP) demonstrated the existence of Mn(III)-intermediates. Furthermore, multicopper oxidase gene amplification provided evidence for the molecular biology of Mn(II) oxidation by strain HY129.
引用
收藏
页数：10
相关论文
共 56 条
[41]   Performance and microbial community of an immobilized biofilm reactor (IBR) for Mn(II)-based autotrophic and mixotrophic denitrification [J].
Su, Jun Feng ;
Zhang, Yuan Ming ;
Liang, Dong Hui ;
Wang, Jia Xing ;
Wang, Zhao ;
Li, Min .
BIORESOURCE TECHNOLOGY, 2019, 286
[42]   Characterization and mechanism of the Cd(II) removal by anaerobic denitrification bacterium Pseudomonas sp. H117 [J].
Su, Junfeng ;
Gao, Chunyu ;
Huang, Tinglin ;
Gao, Yichou ;
Bai, Xuechen ;
He, Lei .
CHEMOSPHERE, 2019, 222 :970-979
[43]   Comparison of denitrification performance by bacterium Achromobacter sp A14 under different electron donor conditions [J].
Su, Junfeng ;
Liang, Donghui ;
Lian, Tingting .
CHEMICAL ENGINEERING JOURNAL, 2018, 333 :320-326
[44]   Degradation of ciprofloxacin by manganese(III) intermediate: Insight into the potential application of permanganate/bisulfite process [J].
Sun, Bo ;
Li, Dan ;
Linghu, Wensheng ;
Guan, Xiaohong .
CHEMICAL ENGINEERING JOURNAL, 2018, 339 :144-152
[45]   Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp S1 [J].
Sun, Zhiyi ;
Lv, Yongkang ;
Liu, Yuxiang ;
Ren, Ruipeng .
BIORESOURCE TECHNOLOGY, 2016, 220 :142-150
[46]   MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].
Tamura, Koichiro ;
Peterson, Daniel ;
Peterson, Nicholas ;
Stecher, Glen ;
Nei, Masatoshi ;
Kumar, Sudhir .
MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739
[47]   DGGE diversity of manganese mine samples and isolation of a Lysinibacillus sp efficient in removal of high Mn (II) concentrations [J].
Tang, Wenwei ;
Gong, Jiemin ;
Wu, Lujun ;
Li, Yanfei ;
Zhang, Mengtian ;
Zeng, Xinping .
CHEMOSPHERE, 2016, 165 :277-283
[48]   Synergistic effects of biogenic manganese oxide and Mn(II)-oxidizing bacterium Pseudomonas putida strain MnB1 on the degradation of 17 α-ethinylestradiol [J].
Thi Nhung Tran ;
Kim, Do-Gun ;
Ko, Seok-Oh .
JOURNAL OF HAZARDOUS MATERIALS, 2018, 344 :350-359
[49]   Spatially resolved characterization of biogenic manganese oxide production within a bacterial biofilm [J].
Toner, B ;
Fakra, S ;
Villalobos, M ;
Warwick, T ;
Sposito, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (03) :1300-1310
[50]   Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria [J].
Wang, Jin ;
Li, Qing ;
Li, Ming-Ming ;
Chen, Tian-Hu ;
Zhou, Yue-Fei ;
Yue, Zheng-Bo .
BIORESOURCE TECHNOLOGY, 2014, 163 :374-376
123456