Lysobacter terricola sp nov., isolated from greenhouse soil

Strain 5GH22-1(T), which was isolated from greenhouse soil in the Yangpyeong region, Gyeonggi province, Republic of Korea, was characterized to be an aerobic, Gram-stain-negative, flagellated, rod -shaped bacterium. It could grow at temperatures from 10 to 33 degrees C (optimum of 28-30 degrees C), in the pH range of 6.0-10.0 (optimum of pH 7.0) and without NaCI. 16S rRNA gene sequence analysis showed that strain 5GH18-14(T) showed the highest sequence similarities with Lysobacter niabensis GH34-4(T) (98.6 %), Lysobacter yangpyeongensis GH19-3(T) (98.1 %), 'Lysobacter fragariae' THG-DN8.7 (97.9 %), Lysobacter terrae THG-A13(T) (97.3 %), 'Lysobacter rhizosphaerae' THG-DN8.3 (97.2 %), 'Lysobacter tyrosinelyticus' THG-DN8.2 (97.2 %) and Lysobacter oryzae YC6269(T) (97.2 %), revealing less than 95.5 % sequence similarities with all other species with validly published names. Phylogenetic trees also indicated that strain 5GH18-14(T) formed a compact subcluster with L. niabensis GH34-4(T), L. yangpyeongensis GH19-3(T), L. terrae THG-A13(T) and L. oryzae YC6269(T) within the genus Lysobacter. The predominant quinone of strain 5GH18-14(T) was Q-8. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine in large amounts, and moderate or small amounts of three unknown phospholipids and two unknown aminophospholipids. DNA-DNA hybridization values with closely related species were below 70 %. The DNA G +C content was 65.9 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it has been demonstrated that strain 5GH18-14(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter terricola sp. nov. is proposed. The type strain is 5GH18-14(T) (=KACC 16954(T)=JCM 30862(T)).