Diagnosis of Epidermolysis Bullosa Acquisita: Multicentre Comparison of Different Assays for Serum Anti-type VII Collagen Reactivity

被引：6

作者：

Holtsche, Maike Marleen ^{[1
]}

van Beek, Nina ^{[1
]}

Hashimoto, Takashi ^{[2
]}

Di Zenzo, Giovanni ^{[3
]}

Zillikens, Detlef ^{[1
]}

Prost-Squarcioni, Catherine ^{[4
]}

Titeux, Matthias ^{[5
]}

Hovnanian, Alain ^{[5
]}

Schmidt, Enno ^{[1
,6
]}

Goletz, Stephanie ^{[6
]}

机构：

[1] Univ Lubeck, Dept Dermatol, Lubeck, Germany

[2] Osaka City Univ, Dept Dermatol, Grad Sch Med, Osaka, Japan

[3] Dermatol Res Hosp, IDI, IRCCS Ist Dermopat Immacolata Ist Ricovero & Cura, Rome, Italy

[4] Univ Paris 13, Referral Ctr AutoImmune Bullous Dis MALIBUL, Dept Dermatol, Avicenne Hosp, Bobigny, France

[5] Univ Paris 05, Imagine Inst Genet Dis, Dept Genet, Paris, France

[6] Univ Lubeck, Lubeck Inst Expt Dermatol, Ratzeburger Allee 160, DE-23538 Lubeck, Germany

来源：

ACTA DERMATO-VENEREOLOGICA | 2021年 / 101卷

关键词：

epidermolysis bullosa acquisita; autoantibody; type VII collagen; BIOCHIP; enzyme-linked immunoassay; immu-noblot;

D O I：

10.2340/00015555-3774

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen noncollagenous domains enzyme-linked immuno assay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, L?beck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP?; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93.1% and specificity of 100%, followed by NC1 BIOCHIP? (sensitivity, 89.1%; specificity, 100%), immunoblotting with human dermis (sensitivity, 87.1%; specificity 100%), NC1-ELISA (sensitivity 82.2%; specificity 98.6%), NC1/NC2 ELISA (sensitivity 88.1%; specificity 93.3%), and full-length type VII collagen ELISA (sensitivity 80.2%; specificity 93.8%).

引用

页数：7

共 41 条

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