Growth, microcystin-production and proteomic responses of Microcystis aeruginosa under long-term exposure to amoxicillin

被引:79
作者
Liu, Ying [1 ]
Chen, Shi [1 ]
Zhang, Jian [1 ]
Gao, Baoyu [1 ]
机构
[1] Shandong Univ, Sch Environm Sci & Engn, Shandong Prov Key Lab Water Pollut Control & Reso, Jinan 250100, Peoples R China
基金
中国国家自然科学基金;
关键词
Combined pollution; Antibiotic toxicity; Semi-continuous culture; Photosynthesis activity; Two-dimensional electrophoresis; Protein protein interaction network; LARGE GENE LISTS; AQUATIC ENVIRONMENT; FUNCTIONAL-ANALYSIS; ANTIOXIDANT SYSTEM; SYNECHOCYSTIS SP; TOXICITY; CYANOBACTERIA; ANTIBIOTICS; STRESS; ERYTHROMYCIN;
D O I
10.1016/j.watres.2016.01.060
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Ecological risk of antibiotics due to the induction of antibiotic-resistant bacteria has been widely investigated, while studies on the hazard of antibiotic contaminants via the regulation of cyanobacteria were still limited. This study focused on the long-term action effect and mechanism of amoxicillin (a broadly used antibiotic) in Microcystis aeruginosa at environmentally relevant concentrations through 30 days of semi-continuous culture. Amoxicillin stimulated the photosynthesis activity and the production of microcystins, and interaction of differential proteins under amoxicillin exposure further manifested the close correlation between the two processes. D1 protein, ATP synthase subunits alpha and beta, enolase, triosephosphate isomerase and phosphoglycerate kinase were candidate target positions of amoxicillin in M. aeruginosa under long-term exposure. Amoxicillin affected the cellular biosynthesis process and the metabolism of carbohydrate and nucleoside phosphate according to the proteomic responses. Under exposure to amoxicillin, stimulated growth rate at the beginning phase and increased production and release of microcystins during the whole exposure period would lead to a higher contamination of M. aeruginosa cells and microcystins, indicating that amoxicillin was harmful to aquatic environments through the promotion of cyanobacterial bloom. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:141 / 152
页数:12
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