Tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. gloriosoides Baker

Floret explant of a local accession of Lilium speciosum Thunb. var, gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophenoxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active charcoal and 170 mg/l NaH2PO4. The rare lily was proliferated in vitro by a scale-bulblet cycling propagation method with a multiplication;ate of eight times at three-month intervals. Finally, 6000 plantlets have been produced within nine months. We established 200 plants in the greenhouse under misty conditions for a four-week period with a 98% survival rate. These plants grew well and elongated with normal flowers in the second year.