Tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. gloriosoides Baker

被引:0
|
作者
Chang, C
Chen, CT
Tsai, YC
Chang, WC [1 ]
机构
[1] Acad Sinica, Inst Bot, Taipei 115, Taiwan
[2] Taoyuan Dist Agr Improvement Stn, Tao Yuan, Taiwan
[3] Taiwan Seed Improvement & Propagat Stn, Taichung, Taiwan
来源
BOTANICAL BULLETIN OF ACADEMIA SINICA | 2000年 / 41卷 / 02期
关键词
bulblet; callus; Lilium speciosum thunb var. gloriosoides Baker; rare plant; scale;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Floret explant of a local accession of Lilium speciosum Thunb. var, gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophenoxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active charcoal and 170 mg/l NaH2PO4. The rare lily was proliferated in vitro by a scale-bulblet cycling propagation method with a multiplication;ate of eight times at three-month intervals. Finally, 6000 plantlets have been produced within nine months. We established 200 plants in the greenhouse under misty conditions for a four-week period with a 98% survival rate. These plants grew well and elongated with normal flowers in the second year.
引用
收藏
页码:139 / 142
页数:4
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