Immuno-PCR Assay for Quantitation of Antibodies to Epstein-Barr Virus

被引：1
|
作者：
Pivovarov, V. D. [1 ]
Ryazantsev, D. Yu. [1 ]
Simonova, M. A. [1 ]
Yegorova, T. V. [1 ]
Khlgatian, S. V. [2 ]
Zavriev, S. K. [1 ]
Svirshchevskaya, E. V. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Mechnikov Inst Vaccines & Sera, Moscow 105064, Russia
来源：
MOLECULAR BIOLOGY | 2018年 / 52卷 / 04期
基金：
俄罗斯科学基金会;
关键词：
quantitative immuno-PCR; ELISA; recombinant proteins; Epstein-Barr virus; EBNA1; IgG1; CROSS-REACT; TECHNOLOGY; INFECTION; DIAGNOSIS; IGG;
D O I：
10.1134/S0026893318040155
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein-Barr virus (EBV) in the human blood serum. EBV nuclear protein 1 fragment (pEBV) was expressed in Escherichia coli. A synthetic single-stranded deoxyribonucleotide was conjugated to streptavidin, and the conjugate was used to detect NEuroEBV-IgG1-biotin complexes by qiPCR. The IgG1 titers determined by qiPCR were compared to the results of enzyme-linked immunosorbent assay (ELISA). The sensitivity of qiPCR was one order of magnitude higher than that of ELISA. Thus, a highly sensitive qiPCR-based assay was developed to quantitate antibodies specific to the recombinant EBV antigen.
引用
收藏
页码：629 / 635
页数：7
相关论文
共 50 条
  • [21] Epstein-Barr virus and multiple sclerosis
    Skliar, A., I
    Torianyk, I. I.
    Osolodchenko, T. P.
    Ponomarenko, S., V
    PATHOLOGIA, 2020, (03): : 390 - 401
  • [22] Epstein-Barr virus and multiple sclerosis
    Salvetti, Marco
    Giovannoni, Gavin
    Aloisi, Francesca
    CURRENT OPINION IN NEUROLOGY, 2009, 22 (03) : 201 - 206
  • [23] A high-throughput neutralizing assay for antibodies and sera evaluation against Epstein-Barr virus
    Zhong, Ling
    Krummenacher, Claude
    Zhang, Wanlin
    Hong, Junping
    Feng, Qisheng
    Zhao, Qinjian
    Chen, Yixin
    Zeng, Mu-Sheng
    Zeng, Yi-Xin
    Xu, Miao
    Zhang, Xiao
    VIROLOGY JOURNAL, 2022, 19 (01)
  • [24] The Association of Epstein-Barr Virus With Cancer
    Patel, Pragnesh D.
    Alghareeb, Rahmah
    Hussain, Afshan
    Maheshwari, Marvi, V
    Khalid, Nabeeha
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2022, 14 (06)
  • [25] Antibodies to Epstein-Barr virus and neurotropic viruses in multiple sclerosis and optic neuritis
    Houen, Gunnar
    Heiden, Julie
    Trier, Nicole H.
    Draborg, Anette H.
    Benros, Michael E.
    Zinkeviciute, Ruta
    Petraityte-Burneikiene, Rasa
    Ciplys, Evaldas
    Slibinskas, Rimantas
    Frederiksen, Jette L.
    JOURNAL OF NEUROIMMUNOLOGY, 2020, 346
  • [26] Serum Epstein-Barr virus DNA load in primary Epstein-Barr virus infection
    Bauer, CC
    Aberle, SW
    Popow-Kraupp, T
    Kapitan, M
    Hofmann, H
    Puchhammer-Stöckl, E
    JOURNAL OF MEDICAL VIROLOGY, 2005, 75 (01) : 54 - 58
  • [27] Longitudinal observation of Epstein-Barr virus antibodies in athletes during a competitive season
    Pottgiesser, Torben
    Schumacher, Yorck Olaf
    Wolfarth, Bernd
    Schmidt-Trucksaess, Arno
    Bauer, Georg
    JOURNAL OF MEDICAL VIROLOGY, 2012, 84 (09) : 1415 - 1422
  • [28] Epstein-Barr virus in tumours
    Anagnostopoulos, I
    Hummel, M
    HISTOPATHOLOGY, 1996, 29 (04) : 297 - 315
  • [29] Clinical significance of anti-Epstein-Barr virus antibodies in systemic chronic active Epstein-Barr virus disease
    Nishio, Miwako
    Saito, Minori
    Yoshimori, Mayumi
    Kumaki, Yuki
    Ohashi, Ayaka
    Susaki, Eri
    Yonese, Ichiro
    Sawada, Megumi
    Arai, Ayako
    FRONTIERS IN MICROBIOLOGY, 2024, 14
  • [30] Epstein-Barr virus.
    Bricaire, F
    ANNALES DE MEDECINE INTERNE, 1997, 148 (05): : 372 - 375
12345