Chemical differentiation of three DMSP lyases from the marine Roseobacter group

被引：23

作者：

Burkhardt, Immo ^{[1
]}

Lauterbach, Lukas ^{[1
]}

Brock, Nelson L. ^{[2
]}

Dickschat, Jeroen S. ^{[1
,2
]}

机构：

[1] Rhein Friedrich Wilhelms Univ Bonn, Kekule Inst Organ Chem & Biochem, Gerhard Domagk Str 1, D-53121 Bonn, Germany

[2] TU Braunschweig, Inst Organ Chem, Hagenring 30, D-38106 Braunschweig, Germany

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 20期

关键词：

RUEGERIA-POMEROYI DSS-3; DIMETHYL SULFIDE; DIMETHYLSULFONIOPROPIONATE LYASE; SUBSTRATE-INHIBITION; BACTERIA; SULFUR; DIMETHYLSULPHONIOPROPIONATE; CATABOLISM; ASSIMILATION; METABOLISM;

D O I：

10.1039/c7ob00913e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Dimethylsulfoniopropionate (DMSP) catabolism of marine bacteria plays an important role in marine and global ecology. The genome of Ruegeria pomeroyi DSS-3, a model organism from the Roseobacter group, harbours no less than three genes for different DMSP lyases (DddW, DddP and DddQ) that catalyse the degradation of DMSP to dimethyl sulfide (DMS) and acrylate. Despite their apparent similar function these enzymes show no significant overall sequence identity. In this work DddQ and DddW from R. pomeroyi and the DddP homolog from Phaeobacter inhibens DSM 17395 were functionally characterised and their substrate scope was tested using several synthetic DMSP analogues. Comparative kinetic assays revealed differences in the conversion of DMSP and its analogues in terms of selectivity and overall velocity, giving additional insights into the molecular mechanisms of DMSP lyases and into their putatively different biological functions.

引用

页码：4432 / 4439

页数：8