Isolation and identification of Clostridium spp. from infections associated with the injection of drugs:: experiences of a microbiological investigation team

被引:36
作者
Brazier, JS [1 ]
Duerden, BI
Hall, V
Salmon, JE
Hood, J
Brett, MM
McLauchlin, J
George, RC
机构
[1] Univ Wales Hosp, PHLS Anaerobe Reference Unit, Cardiff CF14 4XW, S Glam, Wales
[2] Glasgow Royal Infirm, Dept Microbiol, Glasgow G4 0SF, Lanark, Scotland
[3] Cent Publ Hlth Lab, Food Safety Microbiol Lab, London NW9 5DF, England
[4] Cent Publ Hlth Lab, Resp & Syst Infect Lab, London NW9 5DF, England
关键词
D O I
10.1099/0022-1317-51-11-985
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pathogenic species of the genus Clostridium may contaminate the materials used in the injection of drugs and under the right conditions may cause serious or life-threatening disease. C novyi type A was implicated in an outbreak of severe infection with high mortality in injecting drug users who injected heroin extravascularly. The isolation of such highly oxygen-sensitive clostridia from clinical material may require adherence to enhanced methods and, once isolated, commercially available anaerobe identification kits alone may not give an accurate identification. Additional phenotypic tests that are useful in recognising the main pathogenic species are described. Differentiation of C novyi type A from C botulinum type C in reference laboratories was based on 16S rDNA sequence data and specific neutralisation of cytopathic effects in tissue culture.
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收藏
页码:985 / 989
页数:5
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