Increasing the sensitivity of endocervical curettings by performing ThinPrep® Pap on transport container fluid: is diagnostic material going down the drain?

被引:6
作者
Lastra, R. R. [1 ]
Meykler, S. E. [2 ]
Baloch, Z. W. [1 ]
Barroeta, J. E. [2 ]
机构
[1] Hosp Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19107 USA
[2] Penn Hosp, Dept Pathol & Lab Med, Philadelphia, PA 19107 USA
关键词
liquid-based cytology; Pap tests; endocervical curettage; sensitivity; colposcopy; CERVICAL INTRAEPITHELIAL NEOPLASIA; CURETTAGE; PART; GUIDELINES; COLPOSCOPY; MANAGEMENT; WOMEN;
D O I
10.1111/cyt.12177
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objective: The sensitivity of endocervical curettage (ECC) can be suboptimal because of limited epithelial tissue. The false-negative rate for ECC in patients with cervical intraepithelial neoplasia involving the endocervical canal has been reported to be 45%. ECC samples are transported to pathology in formalin- or saline-filled containers; this fluid is discarded after the specimen has been submitted. We evaluated the utility of performing liquid-based cytological preparations from ECC transport container fluid as a way to increase the sensitivity of ECC specimens. Methods: Consecutive ECC specimens received at one of the two participating institutions were selected prospectively. A surgical pathology mesh bag was placed over a ThinPrep (R) CytoLyt (R) solution container, and the specimen was filtered through the bag, collecting the transport fluid in the container. The CytoLyt (R) was processed to obtain a container fluid ThinPrep (R) (CF-TP) liquid-based Papanicolaou (Pap) slide. The CF-TP slides were reviewed and the findings were compared with those from the ECC and follow-up specimens. Results: The cohort included 53 patients. Discrepancies between CF-TP and ECC were seen in 14 of the 53 patients (26%); a more significant lesion was identified in CF-TP relative to ECC in 13 of these cases. CF-TP diagnosis was confirmed in eight of 11 cases with histological follow-up. A positive CF-TP result was confirmed by histology in six of nine cases with negative ECC. Conclusions: Combining the pathological evaluation of ECC specimens with liquid-based cytology performed on the transport container fluid can increase the diagnostic sensitivity of the ECC procedure for the detection of cervical lesions.
引用
收藏
页码:368 / 372
页数:5
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