Two-Step Purification of Cordycepin from Cordyceps millitaris by High-Speed Countercurrent Chromatography

被引:8
作者
Ju, Xiuyun [2 ]
Sun, Yong [2 ]
Cao, Xiaoying [2 ]
Jiang, Jihong [2 ]
Zhang, Tianyou [2 ]
Ito, Yoichiro [1 ]
机构
[1] NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA
[2] Xuzhou Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Peoples R China
关键词
Cordycepin; Cordyceps millitaris; High-speed countercurrent chromatography; HPLC; PREPARATIVE ISOLATION; L;
D O I
10.1080/10826070903188153
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatographic analysis.
引用
收藏
页码:2417 / 2423
页数:7
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