Two-Step Purification of Cordycepin from Cordyceps millitaris by High-Speed Countercurrent Chromatography

被引：8

作者：

Ju, Xiuyun ^{[2
]}

Sun, Yong ^{[2
]}

Cao, Xiaoying ^{[2
]}

Jiang, Jihong ^{[2
]}

Zhang, Tianyou ^{[2
]}

Ito, Yoichiro ^{[1
]}

机构：

[1] NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA

[2] Xuzhou Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2009年 / 32卷 / 16期

关键词：

Cordycepin; Cordyceps millitaris; High-speed countercurrent chromatography; HPLC; PREPARATIVE ISOLATION; L;

D O I：

10.1080/10826070903188153

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatographic analysis.

引用

页码：2417 / 2423

页数：7

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