Two-Step Purification of Cordycepin from Cordyceps millitaris by High-Speed Countercurrent Chromatography

被引：8

作者：

Ju, Xiuyun ^{[2
]}

Sun, Yong ^{[2
]}

Cao, Xiaoying ^{[2
]}

Jiang, Jihong ^{[2
]}

Zhang, Tianyou ^{[2
]}

Ito, Yoichiro ^{[1
]}

机构：

[1] NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA

[2] Xuzhou Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2009年 / 32卷 / 16期

关键词：

Cordycepin; Cordyceps millitaris; High-speed countercurrent chromatography; HPLC; PREPARATIVE ISOLATION; L;

D O I：

10.1080/10826070903188153

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatographic analysis.

引用

页码：2417 / 2423

页数：7

共 50 条

[1] PREPARATIVE SEPARATION OF HIGH-PURITY CORDYCEPIN FROM CORDYCEPS MILITARIS(L.) LINK BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
Xie Huichun
Liang, Yong
Lao, Deqiang
Zhang, Tianyou
Ito, Yoichiro
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2011, 34 (07) : 491 - 499
[2] Purification of yttrium by high-speed countercurrent chromatography
Nakamura, Shigeto
Hashimoto, Hiroyuki
Akiba, Kenichi
Journal of Liquid Chromatography and Related Technologies, 1999, 22 (03): : 451 - 461
[3] Purification of yttrium by high-speed countercurrent chromatography
Nakamura, S
Hashimoto, H
Akiba, K
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1999, 22 (03) : 451 - 461
[4] Supercritical fluid extraction of cordycepin and adenosine from Cordyceps kyushuensis and purification by high-speed counter-current chromatography
Ling, Jian Ya
Zhang, Guo Ying
Lin, Jian Qun
Cui, Zhao Jie
Zhang, Chang Kai
SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 66 (03) : 625 - 629
[5] An efficient two-step approach for the preparative separation and purification of eight polyphenols from Hibiscus manihot L. flower with high-speed countercurrent chromatography
Liu, Ju -Zhao
Wen, Le-Le
Tian, Xiao-Li
Fu, Yu-Jie
Cui, Qi
ARABIAN JOURNAL OF CHEMISTRY, 2023, 16 (06)
[6] SEPARATION AND PURIFICATION OF PEPTIDES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
KNIGHT, M
FAGARASAN, MO
TAKAHASHI, K
GEBLAOUI, AZ
MA, Y
ITO, Y
JOURNAL OF CHROMATOGRAPHY A, 1995, 702 (1-2) : 207 - 214
[7] Purification of Curcumin, Demethoxycurcumin, and Bisdemethoxycurcumin by High-Speed Countercurrent Chromatography
Inoue, Koichi
Nomura, Chihiro
Ito, Sumio
Nagatsu, Akito
Hino, Tomoaki
Oka, Hisao
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (20) : 9328 - 9336
[8] One step purification of corilagin and ellagic acid from Phyllanthus urinaria using high-speed countercurrent chromatography
Jikai, L
Yue, H
Henkel, T
Weber, K
PHYTOCHEMICAL ANALYSIS, 2002, 13 (01) : 1 - 3
[9] Isolation and purification of isoflavones from soy flour by high-speed countercurrent chromatography
Andreas Degenhardt
Peter Winterhalter
European Food Research and Technology, 2001, 213 : 277 - 280
[10] Preparation and purification of epigallocatechin by high-speed countercurrent chromatography (HSCCC)
Cao, XL
Ito, Y
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2004, 27 (01) : 145 - 152

← 1 2 3 4 5 →