Molecular imprinting of AMP by an ionic-noncovalent dual approach

被引：24

作者：

Breton, Florent ^{[1
]}

Delepee, Raphael ^{[1
]}

Agrofoglio, Luigi A. ^{[1
]}

机构：

[1] Univ Orleans, Inst Chim Organ & Analyt, UMR 6005, F-45067 Orleans, France

来源：

JOURNAL OF SEPARATION SCIENCE | 2009年 / 32卷 / 19期

关键词：

Adenosine monophosphate; Molecularly imprinted polymer; Nucleotides; Solid phase extraction; RECOGNITION SITES; MEMBRANES; POLYMERS; 9-(2-PHOSPHONYLMETHOXYETHYL)ADENINE; MECHANISM;

D O I：

10.1002/jssc.200900226

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In order to mimic recognition properties of adenylate kinase, molecularly imprinted polymers (MIPs) were prepared for adenosine 5'-nionophosphate (AMP), a substrate of the enzyme. Different functional monomers interacting with the phosphate moiety were tested, and the MIP giving the best specific binding of AMP was composed with one equivalent of 2-(dimethylamino)ethyl methacrylate and ten equivalents of aciylamide compared to AMP. Packed into solid phase cartridge, this polymer showed similar characteristics than the enzyme, since it was specific for AMP toward other nucleotides.

引用

页码：3285 / 3291

页数：7

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