Programmed cell death induced by (β-D-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells

被引：47
作者：
Chaves, I
Regalado, AP
Chen, M
Ricardo, CP
Showalter, AM
机构：
[1] Inst Tecnol Quim & Biol, Plant Biochem Lab, P-2780901 Oeiras, Portugal
[2] Ohio Univ, Dept Environm & Plant Biol, Mol & Cellular Biol Program, Athens, OH 45701 USA
[3] Inst Super Agron, P-1349017 Lisbon, Portugal
来源：
PHYSIOLOGIA PLANTARUM | 2002年 / 116卷 / 04期
关键词：
D O I：
10.1034/j.1399-3054.2002.1160414.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Programmed cell death (PCD) is involved in plant development and pathogen defence and can be triggered in vitro by several biotic and abiotic stimuli. In this report (beta-D-galactosyl)(3) Yariv reagent, a chemical that specifically binds to arabinogalactan-proteins (AGPs), completely inhibited cell growth and induced PCD in tobacco BY-2 suspension cultured cells. Analysis of DNA from these cells, by agarose gel electrophoresis, revealed a DNA ladder consisting of multimers of 140-170 bp, similar to apoptotic animal DNA internucleosomal fragmentation. Complementary morphological studies revealed additional PCD characteristics in the Yariv-treated BY-2 cells, including cell shrinkage and cytoplasmic condensation. These studies demonstrate the usefulness of BY-2 cells as a model plant PCD system and confirm a link between AGPs and PCD.
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页码：548 / 553
页数：6
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