A specific spectral signature of serum and plasma-derived extracellular vesicles for cancer screening

被引：76

作者：

Krafft, Christoph ^{[1
]}

Wilhelm, Konrad ^{[2
,3
]}

Eremin, Anna ^{[3
]}

Nestel, Sigrun ^{[4
]}

von Bubnoff, Nikolas ^{[5
,6
,7
]}

Schultze-Seemann, Wolfgang ^{[2
]}

Popp, Juergen ^{[1
]}

Nazarenko, Irina ^{[3
]}

机构：

[1] Leibniz Inst Photon Technol, Jena, Germany

[2] Univ Freiburg, Med Ctr, Ctr Surg, Dept Urol, Freiburg, Germany

[3] Univ Freiburg, Fac Med, Med Ctr, Inst Environm Hlth Sci & Hosp Infect Control, Freiburg, Germany

[4] Univ Freiburg, Inst Anat & Cell Biol, Freiburg, Germany

[5] Univ Freiburg, Med Ctr, Dept Hematol Oncol & Stem Cell Transplantat, Freiburg, Germany

[6] DKTK, German Canc Consortium, Heidelberg, Germany

[7] DKFZ, German Canc Res Ctr, Heidelberg, Germany

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2017年 / 13卷 / 03期

关键词：

Nanomedicine; Minimal- and non-invasive diagnostics; Extracellular vesicles; Exosomes; Raman spectroscopy; Infrared spectroscopy; ENHANCED RAMAN-SPECTROSCOPY; PROSTATE;

D O I：

10.1016/j.nano.2016.11.016

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and systemic effects. Being released into body fluids, EV may be used in nanomedicine as a valuable source for diagnostic biomarkers. In this work, infrared and Raman spectroscopy were used for comprehensive comparative analysis of cancer versus non-cancer EV and patient screening. Two different EV fractions enriched in exosomes and microvesicles were isolated by differential centrifugation from serum and plasma of cancer and non-cancer patients and from serum and plasma of a healthy donor. The EV fractions were then subjected to drop-coating deposition and drying on calcium fluoride substrates. Reduction of alpha-helix-rich proteins and enhancement of beta-sheet-rich proteins as a cancer-specific blood EV signature was determined, and subsequently this feature was applied for a pilot study aiming to detect prostate cancer in a test cohort of patients with high-grade prostate carcinoma and benign hypoplasia. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：835 / 841

页数：7

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