Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia)

被引：183
作者：
Thoo, Yin Yin [2 ]
Ho, Swee Kheng [2 ]
Liang, Jia Yun [1 ]
Ho, Chun Wai [1 ]
Tan, Chin Ping [2 ]
机构：
[1] UCSI Univ, Fac Sci Appl, Dept Food Sci & Nutr, Kuala Lumpur 56000, Malaysia
[2] Univ Putra Malaysia, Dept Food Technol, Fac Food Sci & Technol, Upm Serdang 43400, Selangor, Malaysia
来源：
FOOD CHEMISTRY | 2010年 / 120卷 / 01期
关键词：
Mengkudu (Morinda citrifolia); Total phenolic content (TPC); Total flavonoid content (TFC); 2.2 '-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging capacity; 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity; RADICAL SCAVENGING ACTIVITY; IN-VITRO; OPTIMIZATION; LEAVES;
D O I：
10.1016/j.foodchem.2009.09.064
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An investigation into the effects of ethanol concentration (0-100%, v/v), extraction time (20-120 min) and extraction temperature (25-65 degrees C) on the extraction of phenolic antioxidants from mengkudu (Morinda citrifolia) was performed using a single-factor experiment. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used for determination of phenolic compounds. Antioxidant capacity was evaluated by measuring the scavenging effect on 2,2'-azino-bis(3-ethylbinzothiazoline-6-sulphonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals. Experimental results showed that extraction conditions had significant effect on extraction of phenolic compounds and antioxidant capacities. The optimised conditions were 40% ethanol for 80 min at 65 degrees C, with values of 919.95 mg GAE/100 g DW for TPC, 472.73 mg CE/100 g DW for TFC, 791.71 mu mol TEAC/100 g DW for ABTS and 1928.5 mu mol TEAC/100 g DW for DPPH. TPC was significantly correlated with DPPH under the effects of ethanol concentration (r = 0.932) and extraction time (r = -0.938). (C) 2009 Elsevier Ltd. All rights reserved.
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收藏
页码：290 / 295
页数：6
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