Analysis of Diluted Vitreous Samples from Vitrectomy Is Useful in Eyes with Severe Acute Postoperative Endophthalmitis

被引:22
作者
Chiquet, Christophe [1 ]
Maurin, Max [2 ]
Thuret, Gilles [3 ]
Benito, Yvonne [4 ,5 ,6 ]
Cornut, Pierre-Loic [7 ]
Creuzot-Garcher, Catherine [8 ]
Rouberol, Frederic [7 ]
Pechinot, Andre [9 ]
Lina, Gerard [4 ,5 ,6 ]
Romanet, Jean-Paul [1 ]
Bron, Alain [8 ]
Vandenesch, Francois [4 ,5 ,6 ]
机构
[1] Univ Grenoble 1, CHU Grenoble, Univ Hosp, Dept Ophthalmol, F-38043 Grenoble 09, France
[2] Univ Grenoble 1, CHU Grenoble, Dept Microbiol, F-38043 Grenoble 09, France
[3] Univ St Etienne, Univ Hosp, CHU St Etienne, Dept Ophthalmol, St Etienne, France
[4] Univ Lyon 1, Ctr Natl Reference Staphylocoques, Fac Laennec, F-69365 Lyon, France
[5] Hosp Civils Lyon, Bacteriol Lab, Ctr Biol & Pathol Est, Bron, France
[6] INSERM, U851, F-69008 Lyon, France
[7] Univ Lyon 1, Hop Edouard Herriot, Dept Ophthalmol, F-69365 Lyon, France
[8] Univ Dijon, CHU Dijon, Dept Ophthalmol, F-21004 Dijon, France
[9] Univ Dijon, CHU Dijon, Dept Microbiol, F-21004 Dijon, France
关键词
BIOPSY; DIAGNOSIS; CATARACT; SPECTRUM; DNA; PCR;
D O I
10.1016/j.ophtha.2009.06.007
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: This study was designed to compare the diagnostic yield of microbiological analysis performed on diluted and undiluted vitreous samples from pars plana vitrectomy (PPV) in patients with acute postcataract endophthalmitis. Design: Cohort study, evaluation of diagnostic test or technology. Participants: Patients with acute postcataract endophthalmitis (<6 weeks). Methods: Undiluted and diluted vitreous samples were taken from 34 consecutive patients at the beginning of PPV as part of the multicenter prospective study of the French Institutional Endophthalmitis Study (FRIENDS) group. Vitrectomy was performed after 1 (n = 12) or 2 (n = 22) intravitreous antibiotic injections. McNemar's nonparametric test was used to compare culture and polymerase chain reaction (PCR) results between diluted and undiluted samples. Main Outcome Measures: Rate of positivity of conventional culture (brain heart infusion broth) and eubacterial PCR tests from undiluted and diluted vitreous samples. Results: The microbiological analysis of both undiluted and diluted vitreous samples detected and identified a bacterial pathogen in 26 out of 34 cases (76.4%). The analysis of undiluted and diluted vitreous at the time of PPV, using eubacterial PCR and conventional culture, gave similar results (P = 0.99; McNemar test). However, eubacterial PCR was more sensitive than culture in detecting bacteria in vitreous at the time of PPV (76% vs 6%; P = 0.001; McNemar test). The difference in sensitivity between the 2 techniques was primarily associated with false-negative culture results for undiluted samples (2/3 of cases), mainly for coagulase-negative staphylococci. Conclusions: The microbiological results obtained combining PCR and culture techniques were similar for diluted vitreous and undiluted vitreous analysis. When eubacterial PCR is available, sampling diluted vitreous, an easier procedure, may replace sampling undiluted vitreous. Financial Disclosure(s): The authors have no proprietary or commercial interest in any of the materials discussed in this article. Ophthalmology 2009,116:2437-24410 (C) 2009 by the American Academy of Ophthalmology.
引用
收藏
页码:2437 / 2441
页数:5
相关论文
共 18 条
[1]  
[Anonymous], 1995, Arch Ophthalmol, V113, P1479
[2]   Acute postoperative endophthalmitis caused by Staphylococcus lugdunensis [J].
Chiquet, C. ;
Pechinot, A. ;
Creuzot-Garcher, C. ;
Benito, Y. ;
Croize, J. ;
Boisset, S. ;
Romanet, J. P. ;
Lina, G. ;
Vandenesch, F. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :1673-1678
[3]   Eubacterial PCR for bacterial detection and identification in 100 acute postcataract surgery endophthalmitis [J].
Chiquet, Christophe ;
Cornut, Pierre-Loic ;
Benito, Yvonne ;
Thuret, Gilles ;
Maurin, Max ;
Lafontaine, Pierre-Olivier ;
Pechinot, Andre ;
Palombi, Karine ;
Lina, Gerard ;
Bron, Alain ;
Denis, Philippe ;
Carricajo, Anne ;
Creuzot, Catherine ;
Romanet, Jean-Paul ;
Vandenesch, Francois .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2008, 49 (05) :1971-1978
[4]   TREATMENT OF ENDOPHTHALMITIS AFTER CATARACT-EXTRACTION [J].
DOFT, BH ;
KELSEY, SF ;
WISNIEWSKI, S ;
METZ, DJ ;
LOBES, L ;
RINKOFF, J ;
DAVIS, M ;
KASSOFF, A .
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES, 1994, 14 (04) :297-304
[5]   Retinal detachment in the endophthalmitis vitrectomy study [J].
Doft, BM ;
Kelsey, SF ;
Wisniewski, SR .
ARCHIVES OF OPHTHALMOLOGY, 2000, 118 (12) :1661-1665
[6]  
DONAHUE SP, 1993, OPHTHALMOLOGY, V100, P452
[7]   16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates [J].
Drancourt, M ;
Bollet, C ;
Carlioz, A ;
Martelin, R ;
Gayral, JP ;
Raoult, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) :3623-3630
[8]   Molecular diagnosis of infective endocarditis by PCR amplification and direct sequencing of DNA from valve tissue [J].
Gauduchon, V ;
Chalabreysse, L ;
Etienne, J ;
Célard, M ;
Benito, Y ;
Lepidi, H ;
Thivolet-Béjui, F ;
Vandenesch, F .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (02) :763-766
[9]   Microbiologic yields and complication rates of vitreous needle aspiration versus mechanized vitreous biopsy in the endophthalmitis vitrectomy study [J].
Han, DP ;
Wisniewski, SR ;
Kelsey, SF ;
Doft, BH ;
Barza, M ;
Pavan, PR .
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES, 1999, 19 (02) :98-102
[10]  
Han DP, 1996, AM J OPHTHALMOL, V122, P1