Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells

被引：90

作者：

Delacher, Michael ^{[1
,2
,3
,4
]}

Simon, Malte ^{[5
,6
]}

Sanderink, Lieke ^{[1
,2
]}

Hotz-Wagenblatt, Agnes ^{[7
]}

Wuttke, Marina ^{[1
,2
]}

Schambeck, Kathrin ^{[1
,2
]}

Schmidleithner, Lisa ^{[1
,2
]}

Bittner, Sebastian ^{[1
,2
]}

Pant, Asmita ^{[1
,2
]}

Ritter, Uwe ^{[1
,2
]}

Hehlgans, Thomas ^{[1
,2
]}

Riegel, Dania ^{[1
]}

Schneider, Verena ^{[8
,9
]}

Groeber-Becker, Florian Kai ^{[8
,9
]}

Eigenberger, Andreas ^{[10
]}

Gebhard, Claudia ^{[1
]}

Strieder, Nicholas ^{[1
]}

Fischer, Alexander ^{[1
,11
]}

Rehli, Michael ^{[1
,11
]}

Hoffmann, Petra ^{[1
,11
]}

Edinger, Matthias ^{[1
,11
]}

Strowig, Till ^{[12
,14
,15
]}

Huehn, Jochen ^{[13
,15
]}

Schmidl, Christian ^{[1
]}

Werner, Jens M. ^{[16
]}

Prantl, Lukas ^{[10
]}

Brors, Benedikt ^{[6
,17
,18
]}

Imbusch, Charles D. ^{[6
]}

Feuerer, Markus ^{[1
,2
]}

机构：

[1] Regensburg Ctr Intervent Immunol RCI, Regensburg, Germany

[2] Univ Regensburg, Chair Immunol, D-93053 Regensburg, Germany

[3] Univ Med Ctr Mainz, Inst Immunol, D-55131 Mainz, Germany

[4] Univ Med Ctr Mainz, Res Ctr Immunotherapy, D-55131 Mainz, Germany

[5] Heidelberg Univ, Fac Biosci, D-69120 Heidelberg, Germany

[6] German Canc Res Ctr, Div Appl Bioinformat, D-69120 Heidelberg, Germany

[7] German Canc Res Ctr, Core Facil Omics IT & Data Management ODCF, D-69120 Heidelberg, Germany

[8] Univ Hosp Wurzburg, Dept Tissue Engn & Regenerat Med TERM, D-97070 Wurzburg, Germany

[9] Translat Ctr Regenerat Therapies TLZ RT, Fraunhofer Inst Silicate Res ISC, D-97082 Wurzburg, Germany

[10] Univ Hosp Regensburg, Dept Plast Hand & Reconstruct Surg, D-93053 Regensburg, Germany

[11] Univ Hosp Regensburg, Dept Internal Med 3, D-93053 Regensburg, Germany

[12] Helmholtz Ctr Infect Res, Dept Microbial Immune Regulat, D-38124 Braunschweig, Germany

[13] Helmholtz Ctr Infect Res, Dept Expt Immunol, D-38124 Braunschweig, Germany

[14] Hannover Med Sch, D-30625 Hannover, Germany

[15] Hannover Med Sch, Cluster Excellence 2155, RESIST, D-30625 Hannover, Germany

[16] Univ Hosp Regensburg, Dept Surg, D-93053 Regensburg, Germany

[17] Natl Ctr Tumor Dis NCT, D-69120 Heidelberg, Germany

[18] German Canc Res Ctr, German Canc Consortium DKTK, D-69120 Heidelberg, Germany

来源：

IMMUNITY | 2021年 / 54卷 / 04期

基金：

欧洲研究理事会;

关键词：

GENOME BROWSER; POPULATION; ALIGNMENT; LINEAGE; BATF;

D O I：

10.1016/j.immuni.2021.03.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Murine regulatory T (Treg) cells in tissues promote tissue homeostasis and regeneration. We sought to identify features that characterize human Treg cells with these functions in healthy tissues. Single-cell chromatin accessibility profiles of murine and human tissue Treg cells defined a conserved, microbiota-independent tissue-repair Treg signature with a prevailing footprint of the transcription factor BATF. This signature, combined with gene expression profiling and TCR fate mapping, identified a population of tissue-like Treg cells in human peripheral blood that expressed BATF, chemokine receptor CCR8 and HLA-DR. Human BATF(+) CCR8(+) Treg cells from normal skin and adipose tissue shared features with nonlymphoid T follicular helper-like (Tfh-like) cells, and induction of a Tfh-like differentiation program in naive human Treg cells partially recapitulated tissue Treg regenerative characteristics, including wound healing potential. Human BATF(+) CCR8(+) Treg cells from healthy tissue share features with tumor-resident Treg cells, highlighting the importance of understanding the context-specific functions of these cells.

引用

页码：702 / +

页数：36

共 72 条

[1] Regulatory T Cells in Skin Facilitate Epithelial Stem Cell Differentiation
Ali, Niwa
Zirak, Bahar
Rodriguez, Robert Sanchez
Pauli, Mariela L.
Hong-An Truong
Lai, Kevin
Ahn, Richard
Corbin, Kaitlin
Lowe, Margaret M.
Scharschmidt, Tiffany C.
Taravati, Keyon
Tan, Madeleine R.
Ricardo-Gonzalez, Roberto R.
Nosbaum, Audrey
Bertolini, Marta
Liao, Wilson
Nestle, Frank O.
Paus, Ralf
Cotsarelis, George
Abbas, Abul K.
Rosenblum, Michael D.
[J]. CELL, 2017, 169 (06) : 1119 - +
[2] IRF4 instructs effector Treg differentiation and immune suppression in human cancer
Alvisi, Giorgia
Brummelman, Jolanda
Puccio, Simone
Mazza, Emilia M. C.
Tomada, Elisa Paoluzzi
Losurdo, Agnese
Zanon, Veronica
Peano, Clelia
Colombo, Federico S.
Scarpa, Alice
Alloisio, Marco
Vasanthakumar, Ajithkumar
Roychoudhuri, Rahul
Kallikourdis, Marinos
Pagani, Massimiliano
Lopci, Egesta
Novellis, Pierluigi
Blume, Jonas
Kallies, Axel
Veronesi, Giulia
Lugli, Enrico
[J]. JOURNAL OF CLINICAL INVESTIGATION, 2020, 130 (06) : 3137 - 3150
[3] A Distinct Function of Regulatory T Cells in Tissue Protection
Arpaia, Nicholas
Green, Jesse A.
Moltedo, Bruno
Arvey, Aaron
Hemmers, Saskia
Yuan, Shaopeng
Treuting, Piper M.
Rudensky, Alexander Y.
[J]. CELL, 2015, 162 (05) : 1078 - 1089
[4] Induction of Colonic Regulatory T Cells by Indigenous Clostridium Species
Atarashi, Koji
Tanoue, Takeshi
Shima, Tatsuichiro
Imaoka, Akemi
Kuwahara, Tomomi
Momose, Yoshika
Cheng, Genhong
Yamasaki, Sho
Saito, Takashi
Ohba, Yusuke
Taniguchi, Tadatsugu
Takeda, Kiyoshi
Hori, Shohei
Ivanov, Ivaylo I.
Umesaki, Yoshinori
Itoh, Kikuji
Honda, Kenya
[J]. SCIENCE, 2011, 331 (6015) : 337 - 341
[5] Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment
Azizi, Elham
Carr, Ambrose J.
Plitas, George
Cornish, Andrew E.
Konopacki, Catherine
Prabhakaran, Sandhya
Nainys, Juozas
Wu, Kenmin
Kiseliovas, Vaidotas
Setty, Manu
Choi, Kristy
Fromme, Rachel M.
Phuong Dao
McKenney, Peter T.
Wasti, Ruby C.
Kadaveru, Krishna
Mazutis, Linas
Rudensky, Alexander Y.
Pe'er, Dana
[J]. CELL, 2018, 174 (05) : 1293 - +
[6] Bateman A, 2018, METHODS MOL BIOL, V1806, P3, DOI 10.1007/978-1-4939-8559-3_1
[7] Buenrostro JD, 2013, NAT METHODS, V10, P1213, DOI [10.1038/NMETH.2688, 10.1038/nmeth.2688]
[8] A Special Population of Regulatory T Cells Potentiates Muscle Repair
Burzyn, Dalia
Kuswanto, Wilson
Kolodin, Dmitriy
Shadrach, Jennifer L.
Cerletti, Massimiliano
Jang, Young
Sefik, Esen
Tan, Tze Guan
Wagers, Amy J.
Benoist, Christophe
Mathis, Diane
[J]. CELL, 2013, 155 (06) : 1282 - 1295
[9] Integrating single-cell transcriptomic data across different conditions, technologies, and species
Butler, Andrew
Hoffman, Paul
Smibert, Peter
Papalexi, Efthymia
Satija, Rahul
[J]. NATURE BIOTECHNOLOGY, 2018, 36 (05) : 411 - +
[10] Enrichr: interactive and collaborative HTML']HTML5 gene list enrichment analysis tool
Chen, Edward Y.
Tan, Christopher M.
Kou, Yan
Duan, Qiaonan
Wang, Zichen
Meirelles, Gabriela Vaz
Clark, Neil R.
Ma'ayan, Avi
[J]. BMC BIOINFORMATICS, 2013, 14

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