Detection of galectin-3 by novel peptidic photoprobes

被引：17

作者：

van Scherpenzeel, Monique ^{[1
]}

van der Pot, Martin ^{[1
]}

Arnusch, Christopher J. ^{[1
]}

Liskamp, Rob M. J. ^{[1
]}

Pieters, Roland J. ^{[1
]}

机构：

[1] Univ Utrecht, Inst Pharmaceut Sci, Dept Med Chem & Chem Biol, NL-3508 TB Utrecht, Netherlands

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2007年 / 17卷 / 02期

关键词：

galectins; photoprobe; proteomics; peptides;

D O I：

10.1016/j.bmcl.2006.10.043

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：376 / 378

页数：3

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