Embedding thin plant specimens for oriented sectioning

被引:44
作者
Beeckman, T
Viane, R
机构
[1] State Univ Ghent VIB, Dept Genet, B-9000 Ghent, Belgium
[2] Univ Ghent, Dept Biol, Lab Bot, B-9000 Ghent, Belgium
关键词
Arabidopsis root; embedding; orientation; sectioning; plant tissue;
D O I
10.3109/10520290009047981
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Small plant structures such as small primary roots, filamentous mosses and algae are difficult to orient for sectioning since they become wavy and curl during embedding. A method is described for embedding and orienting tiny plant specimens in a glycol methacrylate resin using self-constructed flat molds. Prior to sectioning, small samples can be oriented in both the longitudinal and the transverse plane. As several samples can be sectioned simultaneously, time-consuming trimming of the blocks is reduced substantially. The efficiency of this technique has been demonstrated using the tiny roots of the model plant Arabidopsis thaliana (L.) Heynh.
引用
收藏
页码:23 / 26
页数:4
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