Single-Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

被引：10
作者：
Santiago-Rodriguez, Lenibel
Sanchez-Pomales, Germarie
Cabrera, Carlos R. [1 ]
机构：
[1] Univ Puerto Rico, Dept Chem, San Juan, PR 00936 USA
来源：
ELECTROANALYSIS | 2010年 / 22卷 / 04期
基金：
美国国家科学基金会;
关键词：
Single-walled carbon nanotubes; Self-assembled monolayer; DNA; Electrochemical impedance spectroscopy; DNA hybridization; Nanotubes; Sensors; DEOXYRIBONUCLEIC-ACID; SURFACE; IMMOBILIZATION; HYBRIDIZATION; ELECTROCHEMISTRY; OPTIMIZATION;
D O I：
10.1002/elan.200900319
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A gold surface modified with a self-assembled monolayer of 11-amino-1-undecanethiol (AUT) was used for the covalent immobilization of oxidized single-walled carbon nanotubes (SWNTs). The as-described SWNTs-modified substrate was Subsequently used to attach single-stranded deoxyribonucleic acid (ssDNA) used as a substrate for DNA hybridization. Electrochemical impedance spectroscopy measurements were performed to follow the DNA hybridization process by using the redox Couple [Fe(CN)(6)](3-/4-) as a marker ion. Specifically, changes in charge transfer resistance obtained from the Nyquist Plots were used as the sensing parameter of DNA hybridization. The substrate sensitivity towards changes in target DNA concentration, its selectivity toward different DNA sequences and its reusability are successfully demonstrated in this report.
引用
收藏
页码：399 / 405
页数：7
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