Effect of policosanol from insect wax on amyloid β-peptide-induced toxicity in a transgenic Caenorhabditis elegans model of Alzheimer's disease

被引：19
作者：
Zhang, Xin [1 ]
Ma, Chenjing [1 ]
Sun, Long [1 ]
He, Zhao [1 ]
Feng, Ying [1 ]
Li, Xian [1 ]
Gan, Jin [1 ]
Chen, Xiaoming [1 ]
机构：
[1] Chinese Acad Forestry, Res Inst Resource Insects, Key Lab Cultivating & Utilizat Resource Insects, State Forestry Adm, Kunming 650224, Yunnan, Peoples R China
来源：
BMC COMPLEMENTARY MEDICINE AND THERAPIES | 2021年 / 21卷 / 01期
关键词：
Alzheimer’ s disease; Insect wax; Policosanol; β -Amyloid; C; elegans; CL4176; WHITE WAX; CHOLESTEROL; PROTEIN; POLYSACCHARIDE; HEXACOSANOL; AGGREGATION; INHIBITION; EXPRESSION; SYMPTOMS; APL-1;
D O I：
10.1186/s12906-021-03278-2
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Background Alzheimer's disease (AD), an age-related neurodegenerative disorder and a serious public health concern, is mainly caused by beta-amyloid (A beta)-induced toxicity. Currently, a limited number of drugs are effective against AD, and only a few are used for its treatment. According to traditional Chinese medicine, white wax is mainly composed of policosanol, hexacosanol, and octacosanol. Policosanol has been shown to reduce lipid levels in blood and alleviate the symptoms associated with diabetic complications and neurodegenerative disorders, such as Parkinson's disease and AD. However, the efficacy of policosanol depends on the purity and composition of the preparation, and the therapeutic efficacy of policosanol derived from insect wax (PIW) in AD is unknown. Methods Here, we identified the main components of PIW and investigated the effects of PIW on A beta-induced toxicity and life-span in a transgenic Caenorhabditis elegans model of AD, CL4176. Furthermore, we estimated the expression of amyloid precursor-like protein (apl-1) and the genes involved in various pathways associated with longevity and alleviation of AD-related symptoms in PIW-fed CL4176. Results PIW mainly consists of tetracosanol, hexacosanol, octacosanol, and triacontanol; it could decrease the A beta-induced paralysis rate from 86.87 to 66.97% (P < 0.01) and extend the life-span from 6.2 d to 7.8 d (P < 0.001) in CL4176 worms. Furthermore, PIW downregulated apl-1, a gene known to be associated with the levels of A beta deposits in C. elegans. Additionally, our results showed that PIW modulated the expression of genes associated with longevity-related pathways such as heat shock response, anti-oxidative stress, and glutamine cysteine synthetase. Conclusion Our findings suggest that PIW may be a potential therapeutic agent for the prevention and treatment of AD. However, its effects on murine models and patients with AD need to be explored further.
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页数：12
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共 77 条
[1]   Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway [J].
Abbas, S. ;
Wink, M. .
PHYTOMEDICINE, 2010, 17 (11) :902-909
[2]   Policosanol content and composition in perilla seeds [J].
Adhikari, Prakash ;
Hwang, Keum Taek ;
Park, Jae Nam ;
Kim, Choong Ki .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (15) :5359-5362
[3]   Effects of a nutraceutical combination (berberine, red yeast rice and policosanols) on lipid levels and endothelial function randomized, double-blind, placebo-controlled study [J].
Affuso, F. ;
Ruvolo, A. ;
Micillo, F. ;
Sacca, L. ;
Fazio, S. .
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES, 2010, 20 (09) :656-661
[4]   Scientific Opinion on the substantiation of health claims related to beta-glucans from oats and barley and maintenance of normal blood LDL-cholesterol concentrations (ID 1236, 1299), increase in satiety leading to a reduction in energy intake (ID 851, 852), reduction of post-prandial glycaemic responses (ID 821, 824), and "digestive function" (ID 850) pursuant to Article 13(1) of Regulation (EC) No 1924/2006 EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) [J].
Agostoni, Carlo ;
Bresson, Jean-Louis ;
Fairweather-Tait, Susan ;
Flynn, Albert ;
Golly, Ines ;
Korhonen, Hannu ;
Lagiou, Pagona ;
Lovik, Martinus ;
Marchelli, Rosangela ;
Martin, Ambroise ;
Moseley, Bevan ;
Neuhaeuser-Berthold, Monika ;
Przyrembel, Hildegard ;
Salminen, Seppo ;
Sanz, Yolanda ;
Strain, Sean ;
Strobel, Stephan ;
Tetens, Inge ;
Tome, Daniel ;
van Loveren, Hendrik ;
Verhagen, Hans .
EFSA JOURNAL, 2011, 9 (06)
[5]   SKN-1 links C-elegans mesendodermal specification to a conserved oxidative stress response [J].
An, JH ;
Blackwell, TK .
GENES & DEVELOPMENT, 2003, 17 (15) :1882-1893
[6]   Therapeutics of Alzheimer's disease: Past, present and future [J].
Anand, R. ;
Gill, Kiran Dip ;
Mahdi, Abbas Ali .
NEUROPHARMACOLOGY, 2014, 76 :27-50
[7]   Antiplatelet effects of policosanol (20 and 40 mg/day) in healthy volunteers and dyslipidaemic patients [J].
Arruzazabala, ML ;
Molina, V ;
Mas, R ;
Fernández, L ;
Carbajal, D ;
Valdés, S ;
Castaño, G .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2002, 29 (10) :891-897
[8]   The glutamatergic system and Alzheimer's disease - Therapeutic implications [J].
Butterfield, DA ;
Pocernich, CB .
CNS DRUGS, 2003, 17 (09) :641-652
[9]   Effect of policosanol on platelet aggregation and serum levels of arachidonic acid metabolites in healthy volunteers [J].
Carbajal, D ;
Arruzazabala, ML ;
Valdés, S ;
Más, R .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1998, 58 (01) :61-64
[10]   Echinacoside, a phenylethanoid glycoside from Cistanche deserticola, extends lifespan of Caenorhabditis elegans and protects from Aβ-induced toxicity [J].
Chen, Wei ;
Lin, Hong-Ru ;
Wei, Cong-Min ;
Luo, Xiao-Hua ;
Sun, Meng-Lu ;
Yang, Zhen-Zhou ;
Chen, Xin-Yan ;
Wang, Hong-Bing .
BIOGERONTOLOGY, 2018, 19 (01) :47-65
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