One-step White Blood Cell Separation from Whole Blood On a Centrifugal Microfluidic Device

In this work, we propose a fully automated WBCs isolation device from biological sample utilizing centrifugal microfluidics on a polymer based CD platform. Using the novel Laser Irradiated Ferrowax Microvalves (LIFM) and liquid density gradient medium on CD platform, the total process of a blood sample loading on the density gradient medium, fractionating to concentrate the WBC, decanting plasma layer, and WBC isolation was finished within 5 minutes with only manual step of adding diluted 100 mu L of whole blood. The hematocytometry results showed that the number of WBC was as good as the sample prepared in conventional manual method. Furthermore, As a CD could prepare maximum 24 samples the automatic method is more useful for the high throughput cell isolation as well as enables one to enhance reproducibility, laborious and time-consuming process for the RNA purification.