Study of catalase electrode for organic peroxides assays

被引：21

作者：

Horozova, E ^{[1
]}

Dimcheva, N ^{[1
]}

Jordanova, Z ^{[1
]}

机构：

[1] Paisij Hilendarski Univ Plovdiv, Dept Phys Chem, BG-4000 Plovdiv, Bulgaria

来源：

BIOELECTROCHEMISTRY | 2002年 / 58卷 / 02期

关键词：

immobilized catalase; nafion; graphite; organic-phase enzyme electrode;

D O I：

10.1016/S1567-5394(02)00153-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The catalytic activity of immobilized catalase (EC 1.11.1.6) for two model peroxide compounds (dibenzoyl peroxide and 3-chloroperoxibenzoic acid) in a non-aqueous medium was used to prepare an organic-phase enzyme electrode (OPEE). The enzyme was immobilized within a polymeric film on spectrographic graphite. The amperometric signal of the enzyme electrode in substrate solutions was found to be due to the reduction of oxygen generated in the enzyme layer. The electrode response is proportional to peroxide concentrations up to about 40 muM within the potential range from -450 to -650 mV (vs. Ag/AgCl), and the response time is at most 90 s. The enzyme electrode retains about 35% of its initial activity after a 3-week storage at room temperature. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：181 / 187

页数：7