Methylphosphonate LNA: A locked nucleic acid with a methylphosphonate linkage

被引：8
作者：
Lauritsen, A
Dahl, BM
Dahl, O
Vester, B
Wengel, J
机构：
[1] Univ So Denmark, Nucle Acid Ctr, Dept Chem, DK-5230 Odense M, Denmark
[2] Univ Copenhagen, Dept Chem, DK-2100 Copenhagen, Denmark
[3] Univ So Denmark, Nucle Acid Ctr, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 02期
关键词：
D O I：
10.1016/S0960-894X(02)00882-X
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3-exonucleolytic degradation. (C) 2002 Elsevier Science Ltd. All rights reserved.
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页码：253 / 256
页数：4
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