Transforming growth factor-β1 promotes homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury

被引：1
作者：
Si, Xiaoyun [1 ]
Liu, Ximing [2 ]
Li, Jingjing [1 ]
Wu, Xiaoyan [1 ]
机构：
[1] Wuhan Univ, Zhongnan Hosp, Dept Nephrol, Wuhan 430071, Hubei Province, Peoples R China
[2] Wuhan Gen Hosp Guangzhou Command, Dept Orthopaed Surg, Wuhan 430070, Hubei Province, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 10期
关键词：
Transforming growth factor-beta 1; homing; bone marrow mesenchymal stem cells; renal ischemia-reperfusion injury; SDF-1/CXCR4; axis; IN-VITRO; MODEL; PHENOTYPE; FIBROSIS; RECOVERY; GROWTH; CANCER;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Backgrounds: Acute ischemia reperfusion-induced kidney injury is a common cause of acute renal failure, and it is also an important cause of delayed recovery of transplanted kidney functions and even loss of function. However, there is no effective treatment method in clinical applications presently. Objective: The objective was to investigate effects of transforming growth factor-beta 1 on homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury. Methods: Effects of TGF-beta 1 over-expression in MSCs on expression of CXCR4 and chemotactic effect to SDF-1 were investigated by in vitro transmembrane chemotaxis. Anti-TGF-beta 1 antibody was incubated with ischemia reperfusion injury renal tissue homogenate and effects of anti-TGF-beta 1 antibody were observed. In addition, effects of TGF-beta 1 gene transfection and anti-CXCR4 antibody treatment in MSCs on expression of SDF-1/CXCR4 axis of renal tissues and damage repair were further explored. Results: Expression of TGF-beta 1 mRNA in the IRI group increased significantly, and MSCs transplantation could enhance expression of CXCR4 mRNA in rats of the IRI group, the expression of CXCR4 can be decreased by the anti-TGF-beta 1 antibody and the anti-CXCR4 antibody. TGF-beta 1 induced homing of MSCs in repair of renal ischemic reperfusion injury by regulating expression of CXCR4 on cell membranes. Blue fluorescence of DAPI-positive MSCs cells of renal parenchyma in the IRI+MSC group was enhanced significantly, which was significantly inhibited by anti-TGF-beta 1 and anti-CXCR4 antibody, and the inhibitory effect of anti-CXCR4 antibody was more obvious than that of anti-TGF-beta 1 antibody. Conclusion: Transforming growth factor-beta 1 promotes homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury, which will provide useful data on role of TGF-beta 1 in regulating SDF-1/CXCR4 axis-induced MSCs homing.
引用
收藏
页码：12368 / 12378
页数：11
相关论文
共 50 条
[31]   Intraparenchymal Injection of Bone Marrow Mesenchymal Stem Cells Reduces Kidney Fibrosis After Ischemia-Reperfusion in Cyclosporine-Immunosuppressed Rats [J].
Alfarano, C. ;
Roubeix, C. ;
Chaaya, R. ;
Ceccaldi, C. ;
Calise, D. ;
Mias, C. ;
Cussac, D. ;
Bascands, J. L. ;
Parini, A. .
CELL TRANSPLANTATION, 2012, 21 (09) :2009-2019
[32]   Mesenchymal stem cells induce epithelial mesenchymal transition in melanoma by paracrine secretion of transforming growth factor- [J].
Lv, Chuan ;
Dai, Haiying ;
Sun, Mengyan ;
Zhao, Hui ;
Wu, Kai ;
Zhu, Ji ;
Wang, Yuchong ;
Cao, Xian ;
Xia, Zhaofan ;
Xue, Chunyu .
MELANOMA RESEARCH, 2017, 27 (02) :74-84
[33]   The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury [J].
Yan, Xue-Tao ;
Cheng, Xiao-Li ;
He, Xiang-Hu ;
Zheng, Wen-Zhong ;
Xiao-Fang, Yuan ;
Hu, Chen .
BMC GASTROENTEROLOGY, 2019, 19 (1)
[34]   Mesenchymal stem cells promote pancreatic adenocarcinoma cells invasion by transforming growth factor-β1 induced epithelial-mesenchymal transition [J].
Zhou, Hai-Sen ;
Su, Xiao-Fang ;
Fu, Xing-Li ;
Wu, Guo-Zhong ;
Luo, Kun-Lun ;
Fang, Zheng ;
Yu, Feng ;
Liu, Hong ;
Hu, Hong-Juan ;
Chen, Liu-Sheng ;
Cai, Bing ;
Tian, Zhi-Qiang .
ONCOTARGET, 2016, 7 (27) :41294-41305
[35]   Atorvastatin Improves Survival of Implanted Stem Cells in a Rat Model of Renal Ischemia-Reperfusion Injury [J].
Cal, Jieru ;
Yu, Xiaofang ;
Zhang, Bingying ;
Zhang, Hui ;
Fang, Yi ;
Liu, Shaopeng ;
Liu, Tongqiang ;
Ding, Xiaoqiang .
AMERICAN JOURNAL OF NEPHROLOGY, 2014, 39 (06) :466-475
[36]   Treatment of Femoral Head Necrosis With Bone Marrow Mesenchymal Stem Cells Expressing Inducible Hepatocyte Growth Factor [J].
Pan, Zhi-Min ;
Zhang, Yu ;
Cheng, Xi-Gao ;
Gao, Gui-Cheng ;
Wang, Xiang-Rui ;
Cao, Kai .
AMERICAN JOURNAL OF THERAPEUTICS, 2016, 23 (06) :E1602-E1611
[37]   The Efficacy of Mesenchymal Stem Cells in Therapy of Acute Kidney Injury Induced by Ischemia-Reperfusion in Animal Models [J].
Zhou, Tianbiao ;
Liao, Chunling ;
Lin, Shujun ;
Lin, Wenshan ;
Zhong, Hongzhen ;
Huang, Shuangyi .
STEM CELLS INTERNATIONAL, 2020, 2020
[38]   Homing of bone marrow mesenchymal stem cells mediated by sphingosine 1-phosphate contributes to liver fibrosis [J].
Li, Changyong ;
Kong, Yaxian ;
Wang, Hong ;
Wang, Shuling ;
Yu, Hao ;
Liu, Xin ;
Yang, Lin ;
Jiang, Xiangming ;
Li, Lingsong ;
Li, Liying .
JOURNAL OF HEPATOLOGY, 2009, 50 (06) :1174-1183
[39]   Bone marrow-derived mesenchymal stem cells attenuate myocardial ischemia–reperfusion injury via upregulation of splenic regulatory T cells [J].
Ling-Xiao Pang ;
Wen-Wei Cai ;
Qian Li ;
Heng-Jie Li ;
Min Fei ;
Yong-Sheng Yuan ;
Bin Sheng ;
Ke Zhang ;
Rong-Cheng An ;
Ying-Wei Ou ;
Wen-Jie Zeng .
BMC Cardiovascular Disorders, 21
[40]   The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits [J].
Wang, Yan ;
Li, Yigang ;
Song, Lei ;
Li, Yanyan ;
Jiang, Shan ;
Zhang, Song .
MOLECULAR MEDICINE REPORTS, 2016, 14 (01) :234-242
12345