Original Neuron specific enolase promotes tumor metastasis by activating the Wnt/B-catenin pathway in small cell lung cancer

被引：22

作者：

Zha, Zhiqiang ^{[1
]}

Li, Dailing ^{[1
]}

Zhang, Peiling ^{[1
]}

Wang, Peipei ^{[2
]}

Fang, Xisheng ^{[1
,2
]}

Liu, Xia ^{[1
,2
]}

Weng, Chengyin ^{[1
,2
]}

Li, Baoxiu ^{[1
,2
]}

Wu, Yong ^{[1
,2
]}

Mao, Haibo ^{[1
,2
]}

Wang, Lin ^{[1
,2
]}

Xu, Lin ^{[1
,2
]}

Dong, Jiaming ^{[2
]}

Guan, Mingmei ^{[1
,2
]}

Lu, Lin ^{[1
,2
]}

Liu, Guolong ^{[1
,2
]}

机构：

[1] South China Univ Technol, Guangzhou Peoples Hosp 1, Sch Med, Dept Med Oncol, Guangzhou 510180, Guangdong, Peoples R China

[2] Guangzhou Med Univ, Guangzhou Peoples Hosp 1, Dept Med Oncol, Guangzhou 510180, Guangdong, Peoples R China

来源：

TRANSLATIONAL ONCOLOGY | 2021年 / 14卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Small cell lung cancer; Neuron-specific enolase; Metastasis; Epithelial-mesenchymal transition; EPITHELIAL-MESENCHYMAL TRANSITION; E-CADHERIN; DISEASE EXTENT; EXPRESSION; MIGRATION; INVASION; THERAPY; MARKERS; GROWTH; SLUG;

D O I：

10.1016/j.tranon.2021.101039

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Neuron-specific enolase (NSE) has been used as a specific biomarker for small cell lung cancer (SCLC) patients. Nevertheless, the biological function and mechanism of NSE in SCLC are still unclear. In this study, we clarified the role of NSE in the progression of SCLC and found that NSE expression was positively correlated with distant metastasis. Functional analysis showed that overexpression of NSE promoted migration and invasion of SCLC cells. Mechanism analysis showed that NSE overexpression induced epithelial-mesenchymal transition (EMT) of SCLC cells. Moreover, overexpression of NSE increased the protein expression of -catenin and its downstream target genes, and silencing -catenin eliminated NSE-mediated cell migration, invasion and EMT process. Furthermore, NSE interacted with -catenin and inhibited the degradation of -catenin. Besides, the animal experiments also indicated that NSE could promote the EMT process and distant metastasis of SCLC cells in vivo. In summary, our results revealed that NSE could promote the EMT process of SCLC cells by activating the Wnt/-catenin signaling pathway, thereby promoting cell migration, invasion and distant metastasis, which might serve as a potential target for the therapy of SCLC patients.

引用

页数：11

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