Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections

被引：109

作者：

Manoni, Fabio ^{[2
]}

Fornasiero, Lucia ^{[2
]}

Ercolin, Mauro ^{[2
]}

Tinello, Agostino ^{[2
]}

Ferrian, Melissa ^{[2
]}

Hoffer, Paolo ^{[2
]}

Valverde, Sara ^{[1
]}

Gessoni, Gianluca ^{[1
]}

机构：

[1] Civil Hosp, Serv Lab Med, I-30015 Venice, Italy

[2] Civil Hosp, Serv Lab Med, I-35043 Padua, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 65卷 / 02期

关键词：

Urinary tract infection; Rapid diagnosis; Bacteriuria; Pyuria; UF-1000i; DIAGNOSIS; CULTURE;

D O I：

10.1016/j.diagmicrobio.2009.06.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Because urinary tract infections (UTIs) are a quite common disease, the gold standard for diagnosing UTIs is still bacterial culture, although a large percentage of samples are negative: unnecessary Cultures call be reduced by means of ail effective screening test. The analytic performance of a new urine cytometer, the UF-1000i, has been tested oil 1463 urine samples Submitted to our laboratory for culture. Bacteria and leukocyte counts have been compared by means of the UF-1000i with colony-forriling unit (CFU) quantification on citrate lactose electrolytes deficient agar to assess the best cutoff values. By using quantitative cultures and considering as positive a sample with 10 x 10(5) CFU/mL, 546 positive samples (37%) were observed. If compared with 10 x 10(5) CFU/mL, the cutoff values obtained were 125 bacteria/mu L and 40 leukocytes/mu L, respectively. Analytic parameters Such as sensitivity, specificity, positive predictive value, negative predictive value, and correctly classified incidence were satisfactory. Based oil the results obtained in this study, when using the UF-1000i analyzer for a screening test for UTI, a Cutoff value of 40 white blood cells/mu L should be adopted. The cutoff value for bacteria should be 125/mu L for those clinical conditions in which 10 x 10(5) CFU/mL indicates a positivity. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：103 / 107

页数：5

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