Integrating Near-Infrared Visual Fluorescence with a Photoelectrochemical Sensing System for Dual Readout Detection of Biomolecules

被引:51
作者
Han, Qingzhi [1 ]
Zhao, Xuan [1 ]
Na, Na [1 ]
Ouyang, Jin [1 ]
机构
[1] Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Theoret & Computat Photochem, Beijing 100875, Peoples R China
基金
中国国家自然科学基金;
关键词
Infrared devices - Photoelectrochemical cells - Biomolecules - Indium compounds - Antigens - Copper compounds - Antibodies;
D O I
10.1021/acs.analchem.0c04802
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Compared with traditional visible light-driven fluorescence visualization (FV), near-infrared (NIR)-induced FV is an interesting and promising method, while photoelectrochemical (PEC) immunoassay sensing possesses the advantages of high sensitivity, low cost, and simple instrumentation. We combined PEC sensing with NIR-induced FV together and developed a dual readout sensing platform. In this protocol, based on the antibody-analyte (i.e., antigen, DNA, and RNA) reaction and the sandwich-type structure, CuInS2 microflowers as the matrix provided the original background photocurrent; chlorin e6 (Ce6) was conjugated to antibody-modified upconversion nanoparticles and formed a signal label for the PEC sensing and naked-eye readout. Different from traditional PEC immunosensors, under NIR illumination, the developed dual mode sensing platform could achieve quick qualitative analysis and quantitative analysis. Preliminary application performance of the proposed biosensor in prostate-specific antigen analysis is acceptable, indicating its promising potential in clinical/biological studies.
引用
收藏
页码:3486 / 3492
页数:7
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