Leptin expression in mandarin fish Siniperca chuatsi (Basilewsky): Regulation by postprandial and short-term fasting treatment

被引：35
作者：
Yuan, Xiaochen [1 ,2 ]
Li, Aixuan [1 ,2 ]
Liang, Xu-Fang [1 ,2 ]
Huang, Wei [1 ,2 ]
Song, Yi [1 ,2 ]
He, Shan [1 ,2 ]
Cai, Wenjing [1 ,2 ]
Tao, Ya-xiong [3 ]
机构：
[1] Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Anim Breeding, Minist Agr, Wuhan 430070, Hubei, Peoples R China
[2] Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Hubei, Peoples R China
[3] Auburn Univ, Dept Anat Physiol & Pharmacol, Coll Vet Med, Auburn, AL 36849 USA
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2016年 / 194卷
基金：
中国国家自然科学基金; “十二五”国家科技支撑计划重点项目”;
关键词：
Gene structure; Intron loss; Tissue expression; Postprandial; Energy regulation; MESSENGER-RNA EXPRESSION; FOOD-INTAKE; MOLECULAR-CLONING; GENE-EXPRESSION; GENOMIC CHARACTERIZATION; RECEPTOR EXPRESSION; PLASMA LEPTIN; EVOLUTION; INSULIN; ACID;
D O I：
10.1016/j.cbpa.2016.01.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Most fish species possess duplicate leptin genes (LEP). Mandarin fish (Siniperca chuatsi) leptin A gene (sLEP-A) have been cloned in the previous study. In the present study, we cloned and characterized leptin B gene (sLEP-B) in mandarin fish, including a 471 bp open reading frame (ORF) encoding a 158-amino acid protein. The three-dimensional (3D) structural model of sLEP-B protein showed a highly conserved of tertiary structure similar to that of other vertebrates. Genomic sequencing results indicated that sLEP-B possessed only one intron. This is the first report of the loss of an intron in LEP-B in Perciformes. The different distribution patterns of sLEPs suggest different physiological roles of these two genes. The presence of HNF3 beta, a liver-enriched transcription factor, only in sLEP-A indicated abundant expression and metabolic function of sLEP-A in the liver. In an in vivo experiment, the expressions of brain sLEP-A and sLEP-B were observed to increase after a meal. During the short-term fasting, the expressions of sLEPs in mandarin fish brain were decreased significantly. A persistent and significant increase in hepatic sLEP-A expression supported a relationship between leptin and food intake in mandarin fish. These results suggest that sLEP-A plays an important role in the regulation of energy homeostasis in this carnivorous fish, and sLEP-B is probably a specialized gene responsible for the central nervous system (CNS) control of energy regulation. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：8 / 18
页数：11
相关论文
共 59 条
[1]   Role of leptin in the neuroendocrine response to fasting [J].
Ahima, RS ;
Prabakaran, D ;
Mantzoros, C ;
Qu, DQ ;
Lowell, B ;
MaratosFlier, E ;
Flier, JS .
NATURE, 1996, 382 (6588) :250-252
[2]   Central infusion of recombinant ovine leptin normalizes plasma insulin and stimulates a novel hypersecretion of luteinizing hormone after short-term fasting in mature beef cows [J].
Amstalden, M ;
Garcia, MR ;
Stanko, RL ;
Nizielski, SE ;
Morrison, CD ;
Keisler, DH ;
Williams, GL .
BIOLOGY OF REPRODUCTION, 2002, 66 (05) :1555-1561
[3]   Molecular cloning and genomic characterization of novel Leptin-like genes in salmonids provide new insight into the evolution of the Leptin gene family [J].
Angotzi, Anna R. ;
Stefansson, Sigurd O. ;
Nilsen, Tom O. ;
Rathore, Raja M. ;
Ronnestad, Ivar .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2013, 187 :48-59
[4]   Long-term peripheral treatment of immature coho salmon (Oncorhynchus kisutch) with human leptin has no clear physiologic effect [J].
Baker, DM ;
Larsen, DA ;
Swanson, P ;
Dickhoff, WW .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2000, 118 (01) :134-138
[5]   Leptins and leptin receptor expression in the goldfish (Carassius auratus). Regulation by food intake and fasting/overfeeding conditions [J].
Belen Tinoco, Ana ;
Gabriela Nisembaum, Laura ;
Isorna, Esther ;
Jesus Delgado, Maria ;
de Pedro, Nuria .
PEPTIDES, 2012, 34 (02) :329-335
[6]   SATIATION TIME, APPETITE, AND MAXIMUM FOOD INTAKE OF SOCKEYE SALMON (ONCORHYNCHUS-NERKA) [J].
BRETT, JR .
JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1971, 28 (03) :409-&
[7]   The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[8]   The detection of 3 leptin receptor isoforms in crucian carp gill and the influence of fasting and hypoxia on their expression [J].
Cao, Y. -B. ;
Xue, J. L. ;
Wu, L. -Y. ;
Jiang, W. ;
Hu, P. -N. ;
Zhu, J. .
DOMESTIC ANIMAL ENDOCRINOLOGY, 2011, 41 (02) :74-80
[9]   Genome Analysis Reveals Interplay between 5′UTR Introns and Nuclear mRNA Export for Secretory and Mitochondrial Genes [J].
Cenik, Can ;
Chua, Hon Nian ;
Zhang, Hui ;
Tarnawsky, Stefan P. ;
Akef, Abdalla ;
Derti, Adnan ;
Tasan, Murat ;
Moore, Melissa J. ;
Palazzo, Alexander F. ;
Roth, Frederick P. .
PLOS GENETICS, 2011, 7 (04)
[10]   Effect of short-term fasting on plasma concentrations of leptin and other hormones and metabolites in dairy cattle [J].
Chelikani, PK ;
Ambrose, JD ;
Keisler, DH ;
Kennelly, JJ .
DOMESTIC ANIMAL ENDOCRINOLOGY, 2004, 26 (01) :33-48
123456