Measurement of in vitro P-selectin expression by flow cytometry

被引:0
作者
Kennedy, SD
Igarashi, Y
Kickler, TS
机构
[1] JOHNS HOPKINS UNIV, SCH MED, DEPT PATHOL, DIV HEMATOL & COAGULAT, BALTIMORE, MD 21205 USA
[2] UNIV WASHINGTON, BIOMEMBRANE INST, SEATTLE, WA 98195 USA
关键词
coagulation; flow cytometry; integrins; platelet function; P-selectin;
D O I
暂无
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Measurement of in vivo platelet activation is difficult after phlebotomy and during blood processing for analysis. We used flow cytometry to measure platelet surface expression of P-selectin in the presence and absence of trimethylsphingosine (a platelet activation inhibitor) and compared the results with those from the standard methods of preventing in vitro P-selectin expression. Percent activation was calculated as a ratio of mean sample fluorescence to 100% mean fluorescence after phorbol myristate acetate treatment. Twenty-five micromoles per liter of trimethylsphingosine kept in vitro platelet activation below 5% up to 6 hours after collection and below 10% at 24 hours after collection. Trimethylsphingosine failed to prevent platelet activation caused by centrifugation, storage at 4 degrees C, or stimulation with common agonists. Addition of trimethylsphingosine to whole blood was valuable in preventing in vitro platelet activation. This compound promises to be a useful preservative for diagnostic testing of platelet activation.
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页码:99 / 104
页数:6
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