Case Report: Identification of SARS-CoV-2 in Cerebrospinal Fluid by Ultrahigh-Depth Sequencing in a Patient With Coronavirus Disease 2019 and Neurological Dysfunction

被引：15

作者：

Xiang, Pan ^{[1
]}

Xu, Xinmin ^{[2
]}

Lu, Xin ^{[3
]}

Gao, Lili ^{[1
]}

Wang, Huizhu ^{[2
]}

Li, Zhenpeng ^{[3
]}

Xiong, Haofeng ^{[1
]}

Li, Ruihong ^{[2
]}

Xiong, Yanwen ^{[3
]}

Pu, Lin ^{[1
]}

Qin, Tian ^{[3
]}

Jin, Fangfang ^{[2
]}

Ren, Hongyu ^{[3
]}

Li, Chuansheng ^{[1
]}

Yang, Jing ^{[3
]}

Zhang, Ming ^{[1
]}

Gong, Jie ^{[3
]}

Chen, Xiaoping ^{[3
]}

Zheng, Han ^{[3
]}

Tan, Jianbo ^{[1
]}

Sun, Yao ^{[1
]}

Zhao, Fei ^{[3
]}

Hou, Xuexin ^{[3
]}

Liu, Yufeng ^{[1
]}

Guo, Hebing ^{[1
]}

Hao, Jingjing ^{[1
]}

Kan, Biao ^{[3
]}

Zhou, Haijian ^{[3
]}

Wang, Yajie ^{[2
]}

Liu, Jingyuan ^{[1
]}

机构：

[1] Capital Med Univ, Beijing Ditan Hosp, Dept Crit Care Med, Beijing, Peoples R China

[2] Capital Med Univ, Beijing Ditan Hosp, Dept Clin Lab, Beijing, Peoples R China

[3] Natl Inst Communicable Dis Control & Prevent, Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China

来源：

FRONTIERS IN MEDICINE | 2021年 / 8卷

关键词：

SARS-CoV-2; COVID-19; central nervous system; infection; metagenomic next-generation sequencing; COVID-19; BRAIN;

D O I：

10.3389/fmed.2021.629828

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We reported that the complete genome sequence of SARS-Coronavirus-2 (SARS-CoV-2) was obtained from a cerebrospinal fluid (CSF) sample by ultrahigh-depth sequencing. Fourteen days after onset, seizures, maxillofacial convulsions, intractable hiccups and a significant increase in intracranial pressure developed in an adult coronavirus disease 2019 patient. The complete genome sequence of SARS-CoV-2 obtained from the cerebrospinal fluid indicates that SARS-CoV-2 can invade the central nervous system. In future, along with nervous system assessment, the pathogen genome detection and other indicators are needed for studying possible nervous system infection of SARS-CoV-2.

引用

页数：6

共 25 条

[1] Neuroinvasion by human respiratory coronaviruses [J].

Arbour, N ;

Day, R ;

Newcombe, J ;

Talbot, PJ .

JOURNAL OF VIROLOGY, 2000, 74 (19) :8913-8921

[2] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].

Bankevich, Anton ;

Nurk, Sergey ;

Antipov, Dmitry ;

Gurevich, Alexey A. ;

Dvorkin, Mikhail ;

Kulikov, Alexander S. ;

Lesin, Valery M. ;

Nikolenko, Sergey I. ;

Son Pham ;

Prjibelski, Andrey D. ;

Pyshkin, Alexey V. ;

Sirotkin, Alexander V. ;

Vyahhi, Nikolay ;

Tesler, Glenn ;

Alekseyev, Max A. ;

Pevzner, Pavel A. .

JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) :455-477

[3] Cerebrospinal Fluid Features in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Reverse Transcription Polymerase Chain Reaction (RT-PCR) Positive Patients [J].

Bellon, Mathilde ;

Schweblin, Cecilia ;

Lambeng, Nathalie ;

Cherpillod, Pascal ;

Vazquez, Jessica ;

Lalive, Patrice H. ;

Schibler, Manuel ;

Deffert, Christine .

CLINICAL INFECTIOUS DISEASES, 2021, 73 (09) :E3102-E3105

[4] Two patients with acute meningoencephalitis concomitant with SARS-CoV-2 infection [J].

Bernard-Valnet, R. ;

Pizzarotti, B. ;

Anichini, A. ;

Demars, Y. ;

Russo, E. ;

Schmidhauser, M. ;

Cerutti-Sola, J. ;

Rossetti, A. O. ;

Du Pasquier, R. .

EUROPEAN JOURNAL OF NEUROLOGY, 2020, 27 (09) :E43-E44

[5] Neurologic manifestations of nonhospitalized patients with COVID-19 in Wuhan, China [J].

Ding, Hong ;

Yin, Shanye ;

Cheng, Yahong ;

Cai, Yilun ;

Huang, Weishan ;

Deng, Wenjun .

MEDCOMM, 2020, 1 (02) :253-256

[6] First case of SARS-COV-2 sequencing in cerebrospinal fluid of a patient with suspected demyelinating disease [J].

Domingues, Renan Barros ;

Mendes-Correa, Maria Cassia ;

de Moura Leite, Fernando Brunale Vilela ;

Sabino, Ester Cerdeira ;

Salarini, Diego Zanotti ;

Claro, Ingra ;

Santos, Daniel Wagner ;

de Jesus, Jaqueline Goes ;

Ferreira, Noely Evangelista ;

Romano, Camila Malta ;

Soares, Carlos Augusto Senne .

JOURNAL OF NEUROLOGY, 2020, 267 (11) :3154-3156

[7] Neurological associations of COVID-19 [J].

Ellul, Mark A. ;

Benjamin, Laura ;

Singh, Bhagteshwar ;

Lant, Suzannah ;

Michael, Benedict Daniel ;

Easton, Ava ;

Kneen, Rachel ;

Defres, Sylviane ;

Sejvar, Jim ;

Solomon, Tom .

LANCET NEUROLOGY, 2020, 19 (09) :767-783

[8] Neurological Complications of Coronavirus Disease (COVID-19): Encephalopathy [J].

Filatov, Asia ;

Sharma, Pamraj ;

Hindi, Fawzi ;

Espinosa, Patricio S. .

CUREUS JOURNAL OF MEDICAL SCIENCE, 2020, 12 (03)

[9] Multiple organ infection and the pathogenesis of SARS [J].

Gu, J ;

Gong, EC ;

Zhang, B ;

Zheng, J ;

Gao, ZF ;

Zhong, YF ;

Zou, WZ ;

Zhan, J ;

Wang, SL ;

Xie, ZG ;

Zhuang, H ;

Wu, BQ ;

Zhong, HH ;

Shao, HQ ;

Fang, WG ;

Gao, DS ;

Pei, F ;

Li, XW ;

He, ZP ;

Xu, DZ ;

Shi, XY ;

Anderson, VM ;

Leong, ASY .

JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 202 (03) :415-424

[10] Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China [J].

Huang, Chaolin ;

Wang, Yeming ;

Li, Xingwang ;

Ren, Lili ;

Zhao, Jianping ;

Hu, Yi ;

Zhang, Li ;

Fan, Guohui ;

Xu, Jiuyang ;

Gu, Xiaoying ;

Cheng, Zhenshun ;

Yu, Ting ;

Xia, Jiaan ;

Wei, Yuan ;

Wu, Wenjuan ;

Xie, Xuelei ;

Yin, Wen ;

Li, Hui ;

Liu, Min ;

Xiao, Yan ;

Gao, Hong ;

Guo, Li ;

Xie, Jungang ;

Wang, Guangfa ;

Jiang, Rongmeng ;

Gao, Zhancheng ;

Jin, Qi ;

Wang, Jianwei ;

Cao, Bin .

LANCET, 2020, 395 (10223) :497-506

← 1 2 3 →