Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo

被引：220
作者：
Wang, Zack Z. [1 ]
Au, Patrick
Chen, Tong
Shao, Ying
Daheron, Laurence M.
Bai, Hao
Arzigian, Melanie
Fukumura, Dai
Jain, Rakesh K.
Scadden, David T.
机构：
[1] Maine Med Ctr, Res Inst, Scarborough, ME 04074 USA
[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
[3] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Radiat Oncol,Edwin L Steele Lab, Boston, MA 02114 USA
[4] Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
[5] Fudan Univ, Shanghai Huashan Hosp, Dept Hematol, Shanghai 200040, Peoples R China
[6] Harvard Univ, Stem Cell Inst, Cambridge, MA 02138 USA
来源：
NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 03期
关键词：
D O I：
10.1038/nbt1287
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe the differentiation of human embryonic stem (hES) cells into endothelial cells using a scalable two-dimensional method that avoids an embryoid-body intermediate. After transplantation into severe combined immunodeficient (SCID) mice, the differentiated cells contributed to arborized blood vessels that integrated into the host circulatory system and served as blood conduits for 150 d.
引用
收藏
页码：317 / 318
页数：2
相关论文
共 13 条
[1]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[2]   DEVELOPMENT OF MOUSE HEMATOPOIETIC SYSTEM .I. TYPES OF HEMOGLOBIN PRODUCED IN EMBRYONIC YOLK SAC AND LIVER [J].
BARKER, JE .
DEVELOPMENTAL BIOLOGY, 1968, 18 (01) :14-&
[3]   HEMOGLOBIN ONTOGENY DURING NORMAL MOUSE FETAL DEVELOPMENT [J].
BROTHERTON, TW ;
CHUI, DHK ;
GAULDIE, J ;
PATTERSON, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (06) :2853-2857
[4]   Angiogenesis in health and disease [J].
Carmeliet, P .
NATURE MEDICINE, 2003, 9 (06) :653-660
[5]   Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells [J].
Chadwick, K ;
Wang, LS ;
Li, L ;
Menendez, P ;
Murdoch, B ;
Rouleau, A ;
Bhatia, M .
BLOOD, 2003, 102 (03) :906-915
[6]  
Gehling UM, 2000, BLOOD, V95, P3106
[7]   Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers [J].
Itskovitz-Eldor, J ;
Schuldiner, M ;
Karsenti, D ;
Eden, A ;
Yanuka, O ;
Amit, M ;
Soreq, H ;
Benvenisty, N .
MOLECULAR MEDICINE, 2000, 6 (02) :88-95
[8]   NO mediates mural cell recruitment and vessel morphogenesis in murine melanomas and tissue-engineered blood vessels [J].
Kashiwagi, S ;
Izumi, Y ;
Gohongi, T ;
Demou, ZN ;
Xu, L ;
Huang, PL ;
Buerk, DG ;
Munn, LL ;
Jain, RK ;
Fukumura, D .
JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (07) :1816-1827
[9]   Hematopoietic colony-forming cells derived from human embryonic stem cells [J].
Kaufman, DS ;
Hanson, ET ;
Lewis, RL ;
Auerbach, R ;
Thomson, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (19) :10716-10721
[10]   Creation of long-lasting blood vessels [J].
Koike, N ;
Fukumura, D ;
Gralla, O ;
Au, P ;
Schechner, JS ;
Jain, RK .
NATURE, 2004, 428 (6979) :138-139
12