METHODS OF SITE-SPECIFIC LABELING OF RNA WITH FLUORESCENT DYES

被引：43

作者：

Solomatin, Sergey ^{[1
]}

Herschlag, Daniel ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Biochem, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

METHODS IN ENZYMOLOGY, VOL 469: BIOPHYSICAL, CHEMICAL, AND FUNCTIONAL PROBES OF RNA STRUCTURE, INTERACTIONS AND FOLDING, PT B | 2009年 / 469卷

关键词：

SINGLE-MOLECULE; TERTIARY INTERACTIONS; DNA; DYNAMICS; KINETICS; DOCKING; THERMODYNAMICS; COOPERATIVITY; HETEROGENEITY; TETRALOOP;

D O I：

10.1016/S0076-6879(09)69003-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single molecule fluorescence techniques offer unique insights into mechanisms of conformational changes of RNA. Knowing how to make fluorescently labeled RNA molecules and understanding potential limitations of different labeling strategies is essential for successful implementation of single molecule fluorescence techniques. This chapter offers a step by step overview of the process of obtaining RNA constructs ready for single molecule measurements. Several alternative methods are described for each step, and ways of troubleshooting the most common problems, in particular, splinted RNA ligation, are suggested.

引用

页码：47 / 68

页数：22

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