Key Genes in the Melatonin Biosynthesis Pathway with Circadian Rhythm Are Associated with Various Abiotic Stresses

被引:40
作者
Ahn, Hye-Ryun [1 ,2 ]
Kim, Yu-Jin [3 ]
Lim, You-Jin [4 ]
Duan, Shucheng [4 ]
Eom, Seok-Hyun [4 ]
Jung, Ki-Hong [1 ,2 ]
机构
[1] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 17104, South Korea
[2] Kyung Hee Univ, Crop Biotech Inst, Yongin 17104, South Korea
[3] Pusan Natl Univ, Dept Life Sci & Environm Biochem, Miryang 50463, South Korea
[4] Kyung Hee Univ, Coll Life Sci, Dept Hort Biotechnol, Yongin 17104, South Korea
来源
PLANTS-BASEL | 2021年 / 10卷 / 01期
基金
新加坡国家研究基金会;
关键词
abiotic stress; circadian rhythm; gene expression; melatonin biosynthesis; rice; N-ACETYLSEROTONIN METHYLTRANSFERASE; GROWTH-STIMULATING COMPOUND; FAR RED-LIGHT; DROUGHT TOLERANCE; TRYPTAMINE; 5-HYDROXYLASE; HIGH-TEMPERATURES; PLANT-GROWTH; RICE; ARABIDOPSIS; SEROTONIN;
D O I
10.3390/plants10010129
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Melatonin (N-acetyl-5-methoxytryptamine), a well-known animal hormone, is involved in several biological processes including circadian rhythm and the regulation of abiotic stress. A systematic understanding of the circadian regulation of melatonin biosynthesis-related genes has not been achieved in rice. In this study, key genes for all of the enzymes in the melatonin biosynthetic pathway that showed a peak of expression at night were identified by microarray data analysis and confirmed by qRT-PCR analysis. We further examined the expression patterns of the four genes under drought, salt, and cold stresses. The results showed that abiotic stresses, such as drought, salt, and cold, affected the expression patterns of melatonin biosynthetic genes. In addition, the circadian expression patterns of tryptophan decarboxylase (TDC), tryptamine 5-hydroxylase (T5H), and serotonin N-acetyltransferase (SNAT) genes in wild-type (WT) plants was damaged by the drought treatment under light and dark conditions. Conversely, N-acetylserotonin O-methyltransferase (ASMT) retained the circadian rhythm. The expression of ASMT was down-regulated by the rice gigantea (OsGI) mutation, suggesting the involvement of the melatonin biosynthetic pathway in the OsGI-mediated circadian regulation pathway. Taken together, our results provide clues to explain the relationship between circadian rhythms and abiotic stresses in the process of melatonin biosynthesis in rice.
引用
收藏
页码:1 / 14
页数:14
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