Control of Integrin αIIbβ3 Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

The physical properties Of Substrates are known to control cell adhesion via integrin-mediated Signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion Of leukocytes and platelets. In our previous work, We have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive Fibrinogen layer. Furthermore, fibrinogen immobilized on various Surfaces at high density Supports weak cell adhesion Whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in in aggregated multilayered material characterized by low adhesion forces. In contrast, immobilization of fibrinogen at low density produced a single layer in which molecules were directly attached to the solid Surface, resulting in higher adhesion forces. Consistent with their distinct physical properties, low- but not high-density fibrinogen induced strong alpha(IIb)beta(3)-mediated outside-in signaling in platelets, resulting in their spreading. Moreover, while intact fibrin gels induced strong Signaling in platelets, deposition of fibrinogen oil the surface of fibrin resulted in diminished cell signaling. The data suggest that deposition of a multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties or surfaces, which results in reduced force generation and insufficient Signaling. The mechanism whereby circulating fibrinogen alters adhesive propel-ties of Fibrin clots may have important implications for control of thrombus formation and thrombogenicity of biomaterials.