Polymorphism of Lactose Genes in the Dairy Yeasts Kluyveromyces marxianus, Potential Probiotic Microorganisms

被引：5

作者：

Naumova, E. S. ^{[1
]}

Sadykova, A. Zh. ^{[1
]}

Michailova, Yu. V. ^{[1
]}

Naumov, G. I. ^{[1
]}

机构：

[1] State Inst Genet & Select Ind Microorganisms, Moscow 119991, Russia

来源：

MICROBIOLOGY | 2017年 / 86卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

dairy probiotic yeasts; Kluyveromyces marxianus; molecular karyotyping; Southern hybridization; lactose fermentation; LAC genes; SACCHAROMYCES-CEREVISIAE; LACTIS; SELECTION; BACTERIA; STRAINS;

D O I：

10.1134/S0026261717030122

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Molecular karyotyping and Southern blot hybridization were used to investigate chromosomal polymorphism of the LAC genes controlling lactose fermentation in Kluyveromyces marxianus strains isolated from various dairy products and natural sources in Russia and CIS countries. Profound polymorphism of karyotype patterns and accumulation of LAC genes were observed in dairy K. marxianus strains. K. marxianus strains isolated from dairy products intensively fermented lactose at 37 degrees C after one day of cultivation, while non-dairy strains exhibited delayed lactose fermentation or did not ferment it at all. Based on the fermentation tests, twelve K. marxianus strains were selected, which are of interest as potential probiotic microorganisms suitable for further molecular genetic studies and breeding.

引用

页码：363 / 369

页数：7

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