Inhibitory effects of tranilast on the proliferation and functions of human pterygium-derived fibroblasts

被引:28
作者
Isaji, M
Kikuchi, S
Miyata, H
Ajisawa, Y
Araki-Inazawa, K
Tsukamoto, Y
Amano, Y
机构
[1] Kissei Pharmaceut Co Ltd, Discovery Res R&D, Minamiazumi, Nagano 3998304, Japan
[2] W Japan Railway Co, Osaka Railway Hosp, Dept Ophthalmol, Osaka, Japan
关键词
tranilast; pterygium; fibroblasts; proliferation; chemotaxis; collagen gel contraction; collagen synthesis;
D O I
10.1097/00003226-200005000-00021
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose. We studied the possibility that tranilast, an antiallergic and antiproliferative drug, may be beneficial for the treatment of pterygium. Methods. Pterygium-derived cells were identified by immunohistochemical methods. Growth rate of pterygium-derived cells was determined by using a hemocytometer. Chemotaxis was determined in a microchemotaxis chamber. Pterygium-derived cells were cultured on floating collagen gel, and the contracted diameter was measured. Collagen synthesis by pterygium-derived cells was determined by the collagenase digestive method. Tranilast was added to the culture medium at final concentrations of 0, 12.5, 25, 50, and 100 mu g/ml. Results, Pterygium-derived cells were stained with anti-prolylhydroxylase and anti-alpha-smooth muscle actin, and identified as fibroblasts. Tranilast inhibited the proliferation and chemotaxis of pterygium-derived fibroblasts, and the collagen-gel contraction induced by these cells, but it exerted no inhibitory action on collagen synthesis by pterygium-derived fibroblasts. Conclusion, Tranilast may be useful for suppressing the recurrence and, possibly, the development of pterygium.
引用
收藏
页码:364 / 368
页数:5
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