Risk stratification by long non-coding RNAs profiling in COVID-19 patients

被引：34

作者：

Cheng, Jie ^{[1
,2
]}

Zhou, Xiang ^{[3
]}

Feng, Weijun ^{[4
]}

Jia, Min ^{[5
]}

Zhang, Xinlu ^{[6
]}

An, Taixue ^{[7
]}

Luan, Minyuan ^{[8
]}

Pan, Yi ^{[9
,10
]}

Zhang, Shu ^{[11
]}

Zhou, Zhaoming ^{[12
]}

Wen, Lei ^{[13
]}

Sun, Yun ^{[1
,2
]}

Zhou, Cheng ^{[14
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Ren Ji Hosp, Ctr Reprod Med, Shanghai 200135, Peoples R China

[2] Shanghai Key Lab Assisted Reprod & Reprod Genet, Shanghai, Peoples R China

[3] Gen Hosp Cent Theater Command PLA, Dept Anesthesiol, Wuhan, Peoples R China

[4] Fudan Univ, Shanghai Key Lab Med Epigenet, Inst Biomed Sci,Childrens Hosp,Inst Pediat, Int Colab Med Epigenet & Metab,Minist Sci & Techn, Shanghai, Peoples R China

[5] Fudan Univ, Shanghai Canc Ctr, Dept Radiat Oncol, Shanghai, Peoples R China

[6] Southern Med Univ, Nanfang Hosp, Dept Cardiol, Guangzhou, Peoples R China

[7] Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Peoples R China

[8] Qingdao Univ, Affiliated Hosp, Organ Transplantat Ctr, Qingdao, Peoples R China

[9] German Canc Res Ctr, Div Funct Genome Anal, Heidelberg, Germany

[10] Heidelberg Univ, Fac Med Heidelberg, Heidelberg, Germany

[11] Fudan Univ, Shanghai Canc Ctr, Dept Gynecol Oncol, Shanghai, Peoples R China

[12] Southern Med Univ, Sch Publ Hlth, Dept Radiat Med, Guangzhou, Peoples R China

[13] Guangdong Sanjiu Brain Hosp, Dept Oncol, Guangzhou, Peoples R China

[14] Southern Med Univ, Nanfang Hosp, Dept Radiat Oncol, Guangzhou 510515, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2021年 / 25卷 / 10期

基金：

国家重点研发计划; 中国国家自然科学基金; 中国博士后科学基金;

关键词：

COVID-19; lncRNA; pulmonary injury; RNA-seq; transcriptome; IDENTIFICATION;

D O I：

10.1111/jcmm.16444

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic worldwide. Long non-coding RNAs (lncRNAs) are a subclass of endogenous, non-protein-coding RNA, which lacks an open reading frame and is more than 200 nucleotides in length. However, the functions for lncRNAs in COVID-19 have not been unravelled. The present study aimed at identifying the related lncRNAs based on RNA sequencing of peripheral blood mononuclear cells from patients with SARS-CoV-2 infection as well as health individuals. Overall, 17 severe, 12 non-severe patients and 10 healthy controls were enrolled in this study. Firstly, we reported some altered lncRNAs between severe, non-severe COVID-19 patients and healthy controls. Next, we developed a 7-lncRNA panel with a good differential ability between severe and non-severe COVID-19 patients using least absolute shrinkage and selection operator regression. Finally, we observed that COVID-19 is a heterogeneous disease among which severe COVID-19 patients have two subtypes with similar risk score and immune score based on lncRNA panel using iCluster algorithm. As the roles of lncRNAs in COVID-19 have not yet been fully identified and understood, our analysis should provide valuable resource and information for the future studies.

引用

页码：4753 / 4764

页数：12

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