Spectrophotometric determination of L-cysteine by using polyvinylpyrrolidone-stabilized silver nanoparticles in the presence of barium ions

被引：56

作者：

Bamdad, Farzad ^{[1
]}

Khorram, Fateme ^{[1
]}

Samet, Maryam ^{[1
]}

Bamdad, Kourosh ^{[2
]}

Sangi, Mohammad Reza ^{[1
]}

Allahbakhshi, Fateme ^{[1
]}

机构：

[1] Arak Univ, Dept Chem, Fac Sci, Arak 3815688349, Iran

[2] PNU, Dept Biol, Fac Sci, POB 19395-3697, Tehran, Iran

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 161卷

关键词：

Silver nanoparticles; Cysteine; Aggregation; Human plasma; Surface plasmon; CHROMATOGRAPHY MASS-SPECTROMETRY; AMINO-ACIDS; HOMOCYSTEINE; SERUM; GLUTATHIONE; DISEASES; PVP;

D O I：

10.1016/j.saa.2016.02.030

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this article a simple and selective colorimetric probe for cysteine determination using silver nano particles (AgNPs) is described. The determination process was based upon the surface plasmon resonance properties of polyvinylpyrrolidone-stabilized AgNPs. Interaction of AgNPs with cysteine molecules in the presence of barium ions induced a red shift in the surface plasmon resonance (SPR) maximum of AgNPs, as a result of nanoparticle aggregation. Consequently, yellow color of AgNP solution was changed to pink. The linear range for the determination of cysteine was 3.2-82 mu M (R = 0.9965) with a limit of detection equal to 2.8 mu M (3 sigma). The proposed method was successfully applied to the determination of cysteine in human plasma samples. Acceptable recovery results of the spiked samples confirmed the validity of the proposed method. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：52 / 57

页数：6

共 28 条

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