Inkjet-Print Micromagnet Array on Glass Slides for Immunomagnetic Enrichment of Circulating Tumor Cells

被引：27

作者：

Chen, Peng ^{[1
]}

Huang, Yu-Yen ^{[3
]}

Bhave, Gauri ^{[1
]}

Hoshino, Kazunori ^{[2
]}

Zhang, Xiaojing ^{[1
,3
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[2] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA

[3] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA

来源：

ANNALS OF BIOMEDICAL ENGINEERING | 2016年 / 44卷 / 05期

关键词：

Inkjet printing; Micromagnets; Circulating tumor cells; Microfluidic; Immunomagnetic cell separation; CANCER; LITHOGRAPHY; TECHNOLOGY; DEPOSITION; SYSTEM; BLOOD;

D O I：

10.1007/s10439-015-1427-z

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report an inkjet-printed microscale magnetic structure that can be integrated on regular glass slides for the immunomagnetic screening of rare circulating tumor cells (CTCs). CTCs detach from the primary tumor site, circulate with the bloodstream, and initiate the cancer metastasis process. Therefore, a liquid biopsy in the form of capturing and analyzing CTCs may provide key information for cancer prognosis and diagnosis. Inkjet printing technology provides a non-contact, layer-by-layer and mask-less approach to deposit defined magnetic patterns on an arbitrary substrate. Such thin film patterns, when placed in an external magnetic field, significantly enhance the attractive force in the near-field close to the CTCs to facilitate the separation. We demonstrated the efficacy of the inkjet-print micromagnet array integrated immunomagnetic assay in separating COLO205 (human colorectal cancer cell line) from whole blood samples. The micromagnets increased the capture efficiency by 26% compared with using plain glass slide as the substrate.

引用

页码：1710 / 1720

页数：11

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