Gene expression analysis of rat liver epithelial cells in response to thioacetamide

被引:0
|
作者
Park, Joon-Suk
Yeom, Hye-Jung
Jung, Jin-Wook
Hwang, Seung Yong
Lee, Yong-Soon
Kang, Kyung-Sun
机构
[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Publ Hlth, Seoul 151742, South Korea
[2] Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151742, South Korea
[3] Hanyang Univ, Div Mol & Life Sci, Ansan 426791, Gyeonggi Do, South Korea
[4] Genocheck Co Ltd, Ansan 426791, Gyeonggi Do, South Korea
关键词
thioacetamide; toxicogenomics; gene expression; rat liver epithelial cells;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Thioacetamide (TA) is potent haptotoxincant that requires metabolic activation by mixed-function oxidases. Microarray technology, which is massive parallel gene expression profiling in a single hybridization experiment, has provided as a powerful molecular genetic tool for biological system related toxicant. In this study we focus on the use of toxicogenomics for the determination of gene expression analysis associated with hepatotoxicity in rat liver epithelia[ cell line WB-F344 (WB). The WB cells was used to assess the toxic effects of TA. WB cells were exposed to two concentrations of TA-doses which caused 20% and 50% cell death were chosen and the cells exposed for periods of 2 and 24 h. Our data revealed that following the 2-h exposure at the both of doses and 24-h exposure at the low doses, few changes in gene expression were detected. However, after 24-h exposure of the cells to the high concentration, multiple changes in gene expression were observed. TA treatment gave rise predominantly to up-regulation of genes involved in cell cycle and cell death, but down-regulation of genes involves in cell adhesion and calcium ion binding. Exposure of WB cells to higher doses of the TA gave rise to more changes in gene expression at lower exposure times. These results show that TA regulates expression of numerous genes via direct molecular signaling mechanisms in liver cells.
引用
收藏
页码:203 / 206
页数:4
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