Genetic deletion of Cav3.2 T-type calcium channels abolishes H2S-dependent somatic and visceral pain signaling in C57BL/6 mice

被引：15

作者：

Matsui, Kazuki ^{[1
,2
]}

Tsubota, Maho ^{[1
]}

Fukushi, Saaya ^{[1
]}

Koike, Nene ^{[1
]}

Masuda, Hiroshi ^{[1
]}

Kasanami, Yoshihito ^{[1
]}

Miyazaki, Takaya ^{[1
]}

Sekiguchi, Fumiko ^{[1
]}

Ohkubo, Tsuyako ^{[3
]}

Yoshida, Shigeru ^{[4
]}

Mukai, Yutaro ^{[2
]}

Oita, Akira ^{[2
]}

Takada, Mitsutaka ^{[5
]}

Kawabata, Atsufumi ^{[1
]}

机构：

[1] Kindai Univ, Fac Pharm, Lab Pharmacol & Pathophysiol, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan

[2] Natl Cerebral & Cardiovasc Ctr, Dept Pharm, Suita, Osaka 5658565, Japan

[3] Fukuoka Nursing Coll, Fac Nursing, Div Basic Med Sci & Fundamental Nursing, Fukuoka, Fukuoka 8140193, Japan

[4] Kindai Univ, Dept Life Sci, Fac Sci & Engn, Higashiosaka, Osaka 5778502, Japan

[5] Kindai Univ, Fac Pharm, Div Clin Drug Informat, Higashiosaka, Osaka 5778502, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2019年 / 140卷 / 03期

关键词：

Hydrogen sulfide; Ca(v)3.2 T-type calcium channel; Pain; HYPERALGESIA; INVOLVEMENT; CAV3.2; H2S;

D O I：

10.1016/j.jphs.2019.07.010

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We tested whether genetic deletion of Ca(v)3.2 T-type Ca2+ channels abolishes hydrogen sulfide (H2S)-mediated pain signals in mice. In Ca(v)3.2-expressing HEK293 cells, Na2S, an H2S donor, at 100 mM clearly increased Ba2+ currents, as assessed by whole-cell patch-clamp recordings. In wild-type C57BL/6 mice, intraplantar and intracolonic administration of Na2S evoked mechanical allodynia and visceral nociceptive behavior, respectively, which were abolished by TTA-A2, a T-type Ca2+ channel blocker. In Ca(v)3.2-knockout mice of a C57BL/6 background, Na2S caused neither somatic allodynia nor colonic nociception. Our study thus provides definitive evidence for an essential role of Ca(v)3.2 in H2S-dependent somatic and colonic pain. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.

引用

页码：310 / 312

页数：3

共 15 条

[1] TRPA1 Has a Key Role in the Somatic Pro-Nociceptive Actions of Hydrogen Sulfide [J].