Quality control of PET radiopharmaceuticals by high-performance liquid chromatography with tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence detection

被引：3

作者：

Nakao, Ryuji ^{[1
]}

Furutsuka, Kenji ^{[2
]}

Fukumura, Toshimitsu

Yamaguchi, Masatoshi ^{[3
]}

Suzuki, Kazutoshi

机构：

[1] Natl Inst Radiol Sci, Mol Imaging Ctr, Inage Ku, Chiba 2638555, Japan

[2] Sumitomo Accelerator Serv, Tokyo 1418686, Japan

[3] Fukuoka Univ, Fac Pharmaceut Sci, Fukuoka 8140180, Japan

来源：

BIOMEDICAL CHROMATOGRAPHY | 2010年 / 24卷 / 02期

关键词：

PET radiopharmaceuticals; specific radioactivity; quality control; tris(2,2 '-bipyridyl)ruthenium(II); electrogenerated chemiluminescence; TRICYCLIC ANTIDEPRESSANTS; PLASMA; HPLC;

D O I：

10.1002/bmc.1274

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive reversed-phase liquid chromatographic (HPLC) method was investigated to analyze a range of positron emission tomography (PET) radiopharmaceuticals using electrogenerated chemiluminescence (ECL) detection. ECL is based on the reaction of PET molecules with tris(2,2'-bipyridyl)ruthenium(III) [Ru(bpy)(3)(3+)], which is generated through the on-line electro-oxidation of Ru(bpy)(3)(2+). In 21 different radiopharmaceuticals studied, 18 compounds could be detected with detection limits (signal-to-noise ratio = 3) of 0.12-72 ng/mL per 20 mu L injection. Sufficient reproducibility and linearity were obtained for the quantitative determination of PET molecules in pharmaceutical fluid. This method could be successfully applied to quality control tests of PET radiopharmaceuticals with ultra-high specific radioactivity. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：202 / 208

页数：7

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