Bifunctional mechanisms of autophagy and apoptosis regulations in melanoma from Bacillus subtilis natto fermentation extract

被引:38
|
作者
Chou, Hsin-Yu [1 ]
Liu, Li-Heng [2 ]
Chen, Chung-Yi [3 ]
Lin, I-Fan [4 ]
Ali, Daoud [5 ]
Lee, Alan Yueh-Luen [1 ,6 ,7 ]
Wang, Hui-Min David [1 ,8 ,9 ,10 ]
机构
[1] Natl Chung Hsing Univ, Program Tissue Engn & Regenerat Med, Taichung 402, Taiwan
[2] Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10617, Taiwan
[3] Fooyin Univ, Sch Med & Hlth Sci, Kaohsiung 831, Taiwan
[4] Challenge Bioprod Co Ltd, Touliu 640, Yunlin, Taiwan
[5] King Saud Univ, Coll Sci, Dept Zool, BOX 2455, Riyadh 11451, Saudi Arabia
[6] Natl Hlth Res Inst, Natl Inst Canc Res, Miaoli 35053, Taiwan
[7] Kaohsiung Med Univ, Coll Life Sci, Dept Biotechnol, Kaohsiung 80708, Taiwan
[8] Natl Chung Hsing Univ, Grad Inst Biomed Engn, Taichung 402, Taiwan
[9] Kaohsiung Med Univ, Coll Med, Grad Inst Med, Kaohsiung 807, Taiwan
[10] China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung 404, Taiwan
关键词
Bacillus subtilis natto Extract; Melanoma; Oxidative stress; Autophagy; Apoptosis; OXIDATIVE STRESS; AMPK; CANCER; KINASE; LKB1; SOY; ROS;
D O I
10.1016/j.fct.2021.112020
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Melanoma is one of the most dangerous malignant epidermal cancers. Natto freeze-drying extract (NFDE) and natto water extract (NWE) were isolated from natto, soybeans fermented by Bacillus subtilis natto, which were assessed as potential anti-melanoma agents. Cell cytotoxicity assays revealed significant anti-melanoma effects of NFDE and NWE in a dose-dependent manner, and exhibited low influences on normal skin cells, including Hs68, HaCaT and adipose tissue-derived stem cells (ADSCs), respectively. Through a flow cytometer assay and autophagy acridine orange staining, the cellular death phenomenon shifted from autophagy to apoptosis with the increased dosages. Reactive oxygen species (ROS) were enhanced using DCFDA (2,7-dichlorodihydrofluorescein diacetate) staining when melanoma cells were treated with the extract. NFDE and NWE treatments increase the oxidative stress of cancer cells and cause apoptosis by inhibiting AMP-activated protein kinase (AMPK). NFDE and NWE were considered to play a critical role in cell death through ROS adjustment, autophagy regulation and apoptosis promotion.
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页数:11
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