A CdSe/ZnS Core/Shell Quantum Dot-based Fluorescence-linked Immunosorbent Assay for the Sensitive and Accurate Detection of Procalcitonin

被引：5
作者：
Lv, Yanbing [1 ]
Jin, Qiaoli [1 ]
Li, Jinjie [1 ]
Xu, Yanxia [1 ]
Li, Lin Song [1 ]
Shen, Huaibin [1 ]
Wu, Ruili [1 ]
机构：
[1] Henan Univ, Collaborat Innovat Ctr Nano Funct Mat & Applicat, Sch Mat & Engn, Key Lab Special Funct Mat,Minist Educ,Natl & Loca, Kaifeng 475004, Henan, Peoples R China
来源：
CHEMISTRY LETTERS | 2021年 / 50卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Double antibodies coating; Quantum dot-based fluorescence-linked immunosorbent assay (QD-FLISA); C-REACTIVE PROTEIN; IMMUNOASSAY; NANOPARTICLES; DISCRIMINATION; SEPSIS;
D O I：
10.1246/cl.200655
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this study, we used a CdSe/ZnS core/shell quantum dot (QD) as a fluorescent probe and developed a quantum dot-based fluorescence-linked immunosorbent assay (QD-FLISA) to quantitatively determine procalcitonin (PCT) levels in samples. The QD-antibody probe had a high fluorescent intensity and excellent stability, which met the needs of commercial fluorescent probe materials. Due to the excellent properties of clinical testing for PCT, this QD-FLISA method showed tremendous potential for use in in vitro diagnostic (IVD) kits.
引用
收藏
页码：235 / 239
页数：5
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