A CdSe/ZnS Core/Shell Quantum Dot-based Fluorescence-linked Immunosorbent Assay for the Sensitive and Accurate Detection of Procalcitonin

被引：5

作者：

Lv, Yanbing ^{[1
]}

Jin, Qiaoli ^{[1
]}

Li, Jinjie ^{[1
]}

Xu, Yanxia ^{[1
]}

Li, Lin Song ^{[1
]}

Shen, Huaibin ^{[1
]}

Wu, Ruili ^{[1
]}

机构：

[1] Henan Univ, Collaborat Innovat Ctr Nano Funct Mat & Applicat, Sch Mat & Engn, Key Lab Special Funct Mat,Minist Educ,Natl & Loca, Kaifeng 475004, Henan, Peoples R China

来源：

CHEMISTRY LETTERS | 2021年 / 50卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Double antibodies coating; Quantum dot-based fluorescence-linked immunosorbent assay (QD-FLISA); C-REACTIVE PROTEIN; IMMUNOASSAY; NANOPARTICLES; DISCRIMINATION; SEPSIS;

D O I：

10.1246/cl.200655

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we used a CdSe/ZnS core/shell quantum dot (QD) as a fluorescent probe and developed a quantum dot-based fluorescence-linked immunosorbent assay (QD-FLISA) to quantitatively determine procalcitonin (PCT) levels in samples. The QD-antibody probe had a high fluorescent intensity and excellent stability, which met the needs of commercial fluorescent probe materials. Due to the excellent properties of clinical testing for PCT, this QD-FLISA method showed tremendous potential for use in in vitro diagnostic (IVD) kits.

引用

页码：235 / 239

页数：5

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