Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

被引：40

作者：

Jin Ming-hui ^{[1
,2
]}

Tao Jia-hui ^{[1
]}

Li Q ^{[1
]}

Cheng Ying ^{[1
]}

Sun Xiao-xu ^{[2
]}

Wu Kong-ming ^{[2
]}

Xiao Yu-tao ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

来源：

JOURNAL OF INTEGRATIVE AGRICULTURE | 2021年 / 20卷 / 03期

关键词：

Spodoptera frugiperda; ABCC2; CRISPR/Cas9; Bt receptor; Cry1F; HELICOVERPA-ARMIGERA; CRISPR/CAS9; MUTATION; RECEPTOR; CADHERIN; COTTON; MAIZE;

D O I：

10.1016/S2095-3119(19)62772-3

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a SfABCC2 knockout strain of S. frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F. Results from bioassays showed that the SfABCC2 knockout S. frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain, but no resistance to Vip3A toxin from Bt. These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F.

引用

页码：815 / 820

页数：6

共 43 条

[41] On-plant survival and inheritance of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis
Crespo, Andre L. B.
Spencer, Terence A.
Alves, Analiza P.
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Blankenship, Erin E.
Magalhaes, Leonardo C.
Siegfried, Blair D.
PEST MANAGEMENT SCIENCE, 2009, 65 (10) : 1071 - 1081
[42] Expression of a Chimeric Gene Encoding Insecticidal Crystal Protein Cry1Aabc of Bacillus thuringiensis in Chickpea (Cicer arietinum L.) Confers Resistance to Gram Pod Borer (Helicoverpa armigera Hubner.)
Das, Alok
Datta, Subhojit
Thakur, Shallu
Shukla, Alok
Ansari, Jamal
Sujayanand, G. K.
Chaturvedi, Sushil K.
Kumar, P. A.
Singh, N. P.
FRONTIERS IN PLANT SCIENCE, 2017, 8
[43] The First Cry2Ac-Type Protein Toxic to Helicoverpa armigera: Cloning and Overexpression of Cry2ac7 Gene from SBS-BT1 Strain of Bacillus thuringiensis
Saleem, Faiza
Shakoori, Abdul Rauf
TOXINS, 2017, 9 (11):

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