The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land

被引:64
作者
Biscotti, Maria Assunta [1 ]
Gerdol, Marco [2 ]
Canapa, Adriana [1 ]
Forconi, Mariko [1 ]
Olmo, Ettore [1 ]
Pallavicini, Alberto [2 ]
Barucca, Marco [1 ]
Schartl, Manfred [3 ,4 ]
机构
[1] Univ Politecn Marche, Dipartimento Sci Vita & Ambiente, Via Brecce Bianche, I-60131 Ancona, Italy
[2] Univ Trieste, Dipartimento Sci Vita, Via Licio Giorgeri 5, I-34127 Trieste, Italy
[3] Univ Wurzburg, Bioctr, Dept Physiol Chem, D-97074 Wurzburg, Germany
[4] Univ Clin Wurzburg, Comprehens Canc Ctr Mainfranken, D-97078 Wurzburg, Germany
来源
SCIENTIFIC REPORTS | 2016年 / 6卷
关键词
MITOCHONDRIAL GENOME; AUSTRALIAN LUNGFISH; PROTEIN; EXPRESSION; COELACANTH; SURFACTANT; SELECTION; TETRAPOD; SEQUENCES; INFERENCE;
D O I
10.1038/srep21571
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lungfish and coelacanths are the only living sarcopterygian fish. The phylogenetic relationship of lungfish to the last common ancestor of tetrapods and their close morphological similarity to their fossil ancestors make this species uniquely interesting. However their genome size, the largest among vertebrates, is hampering the generation of a whole genome sequence. To provide a partial solution to the problem, a high-coverage lungfish reference transcriptome was generated and assembled. The present findings indicate that lungfish, not coelacanths, are the closest relatives to land-adapted vertebrates. Whereas protein-coding genes evolve at a very slow rate, possibly reflecting a "living fossil" status, transposable elements appear to be active and show high diversity, suggesting a role for them in the remarkable expansion of the lungfish genome. Analyses of single genes and gene families documented changes connected to the water to land transition and demonstrated the value of the lungfish reference transcriptome for comparative studies of vertebrate evolution.
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页数:12
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