Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes

被引:11
作者
Bennett, George N. [1 ,3 ]
San, Ka-Yiu [2 ,3 ]
机构
[1] Rice Univ, Dept BioSci, Houston, TX 77005 USA
[2] Rice Univ, Dept Bioengn, Houston, TX 77005 USA
[3] Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77005 USA
基金
美国国家卫生研究院;
关键词
Escherichia coli; Electron transfer chain; Quinone; Metabolome; Oxygen; Regulation; Enzyme; Competition; ARC 2-COMPONENT SYSTEM; L-VALINE PRODUCTION; ESCHERICHIA-COLI; CORYNEBACTERIUM-GLUTAMICUM; GENE-EXPRESSION; SIGNAL-TRANSDUCTION; COENZYME-Q; TRANSCRIPTIONAL REGULATION; ANAEROBIC REPRESSION; MICROAEROBIC GROWTH;
D O I
10.1007/s10295-016-1851-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microaerobic growth is of importance in ecological niches, pathogenic infections and industrial production of chemicals. The use of low levels of oxygen enables the cell to gain energy and grow more robustly in the presence of a carbon source that can be oxidized and provide electrons to the respiratory chain in the membrane. A considerable amount of information is available on the genes and proteins involved in respiratory growth and the regulation of genes involved in aerobic and anaerobic metabolism. The dependence of regulation on sensing systems that respond to reduced quinones (e.g. ArcB) or oxygen levels that affect labile redox components of transcription regulators (Fnr) are key in understanding the regulation. Manipulation of the amount of respiration can be difficult to control in dense cultures or inadequately mixed reactors leading to inhomogeneous cultures that may have lower than optimal performance. Efforts to control respiration through genetic means have been reported and address mutations affecting components of the electron transport chain. In a recent report completion for intermediates of the ubiquinone biosynthetic pathway was used to dial the level of respiration vs lactate formation in an aerobically grown E. coli culture.
引用
收藏
页码:647 / 658
页数:12
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