A Self-Assembled Protein Nanotube with High Aspect Ratio

被引：67

作者：

Miranda, Frederico F. ^{[1
]}

Iwasaki, Kenji ^{[4
,5
]}

Akashi, Satoko ^{[3
]}

Sumitomo, Koji ^{[6
]}

Kobayashi, Mime ^{[7
]}

Yamashita, Ichiro ^{[4
,7
]}

Tame, Jeremy R. H. ^{[1
]}

Heddle, Jonathan G. ^{[2
]}

机构：

[1] Yokohama City Univ, Prot Design Lab, Tsurumi Ku, Yokohama, Kanagawa, Japan

[2] Tokyo Inst Technol, Global Edge Inst, Midori Ku, Kanagawa 2268501, Japan

[3] Yokohama City Univ, Struct Biol Lab, Tsurumi Ku, Kanagawa 2300045, Japan

[4] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

[5] Osaka Univ, Inst Prot Res, Lab Prot Synth & Express, Osaka, Japan

[6] NTT Basic Res Labs, Kanagawa 2430198, Japan

[7] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan

来源：

SMALL | 2009年 / 5卷 / 18期

关键词：

nanotubes; nanowires; ring proteins; self-assembly; synthetic biology; BINDING ATTENUATION PROTEIN; CARBON NANOTUBES; TRAP; RNA; BIOTEMPLATE; NANOWIRES; VIRUS; CAGES;

D O I：

10.1002/smll.200900667

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Production of a self-assembled protein nanotube achieved through engineering of the 11mer ring protein trp RNA-binding attenuation protein is described. The produced mutant protein is able to stack in solution to produce an extremely narrow, uniform nanotube apparently stabilized by a mixture of disulfide bonds and hydrophobic interactions. Assembly is reversible and the length of tube can potentially be controlled. Large quantities of hollow tubes 8.5 nm in overall diameter with lengths varying from 7 nm to over 1 mu m are produced. The structure is analyzed using transmission electron microscopy, atomic force microscopy, mass spectrometry, and single-particle analysis and it is found that component rings stack in a head-to-head fashion. The internal diameter of the tube is 2.5 nm, and the amino acid residues lining the central cavity can be mutated, raising the possibility that the tube can be filled with a variety of conducting or semiconducting materials.

引用

页码：2077 / 2084

页数：8

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